Comparative genomic hybridization by array of yeast to screen for genes involved in maintaining genome stability in diploid cells

ABSTRACT: The screen for genes involved in the spontaneous loss of heterozygosity mutagenesis (SLM) in diploid cells was done on the collections of deletion strains of Saccharomyces cerevisiae created by the Saccharomyces Genome Deletion Project (http://www-sequence.stanford.edu/group/yeast_deletion_project/). Screens were performed on the pools of clones. Barcode microarrays were used to detect the increased SLM frequency leading to relative increase in abundance of deletion clone in a pool. Collections used were: Homozygous Diploid (HD) and Essential (E) mixed to create single HDE pool. Three mutagenesis markers were used: (1) naturally existing mating type locus, and two newly introduced markers, (2) CAN1/can1delta, (3) URA3/ura3delta.
1. To detect the increased SLM frequency at the mating ty

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Marek Skoneczny 

PROVIDER: E-MEXP-2685 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress