Transcription profiling of human hep2 cells infected with Streptococcus pyogenes over time

ABSTRACT: Two biological replicate experiments were performed to estimate the bias of the gene expression pattern of infected and non-infected HEp-2 cells. Microarrays hybridized with RNA from 2 h of non-infected HEp-2 cells were used as reference chips for the comparison with microarrays hybridized with RNA from 2 h and 4 h of eukaryotic cells exposed to wt-bacteria and .fasX-mutant. As a reference for chips hybridized with RNA prepared from 6 h p. i. and 8 h p. i. of both GAS-infected HEp-2 cells we used chips that were hybridized with RNA isolated from non-infected cells 8 h p. i. We also compared the microarray data from 2 h of non-infected HEp-2 cells with those from 8 h of non-infected HEp-2 cells to determine the influence of the extended culture on the non-infected cells. Only such genes whi

ORGANISM(S): Homo sapiens

DISEASE(S): 2h after infection with wildtype Streptococcus pyogenes

SUBMITTER: Dirk Koczan 

PROVIDER: E-MEXP-271 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress