Transcription profiling by array of Burkholderia cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species

ABSTRACT: B. cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species.
To expose the cultures to heat stress, cells were grown at 37ºC to an O.D. of 0.4 to 0.45 and then transferred into a different shaking incubator at 42.5ºC, incubated for 1 hour at 150 rpm and harvested.
To expose cultures to oxidative stress by reactive oxygen species, cells were grown at 37ºC to an OD of 0.5. Then t butyl hydroperoxide or hydrogen peroxide solution were added to the culture at 0.001% and 0.15% final concentration. The culture was further incubated for 15 min and then harvested.
The expression profiles were compared to cells grown in LB medium without exposure to stress.

ORGANISM(S): Burkholderia cenocepacia

SUBMITTER: Andrea Sass 

PROVIDER: E-MEXP-2791 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress