Transcription profiling by array of mouse spleen in response to egg ovomucoid treatment

ABSTRACT: Thirty female BALB/c mice of 6-8 weeks of age were purchased from Charles River (Montreal, QC, Canada) and divided randomly into control (n=15) and treated (n=15) groups. Mice were maintained on an egg free diet in the pathogen-free conditions at the University of Guelph Central Animal Facility. Animals were maintained between 22–24°C and at a relative humidity of 40–50%. The light-to-dark cycle was maintained at 12 h from 7 AM to 7 PM. All of the animal experiments were approved by the University of Guelph Animal Care Committee according the Canadian Council on Animal Care (CCAC) guidelines.Mice were exposed to intra-gastric gavage (i.g.) twice every week for 5 weeks with OVM (1 mg/100 μl) and 10 μg of cholera toxin adjuvant. An amino acid (Sigma, St. Louis, MO) solution of 10 μg/μl (100

ORGANISM(S): Mus musculus

SUBMITTER: Mainul Husain 

PROVIDER: E-MEXP-2796 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress