Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

15 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of human BeWo trophoblasts stimulated with Coxiella burnetii, the agent of Q fever

ABSTRACT: The transcriptional response of BeWo cells stimulated with C. burnetii was analyzed using microarrays covering the whole human genome.

ORGANISM(S): Homo sapiens

SUBMITTER: Yannick Le Priol

PROVIDER: E-MEXP-2800 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Coxiella burnetii, the agent of Q fever, replicates within trophoblasts and induces a unique transcriptional response.

Ben Amara Amira A Ghigo Eric E Le Priol Yannick Y Lépolard Catherine C Salcedo Suzana P SP Lemichez Emmanuel E Bretelle Florence F Capo Christian C Mege Jean-Louis JL

PloS one 20101214 12

Q fever is a zoonosis caused by Coxiella burnetii, an obligate intracellular bacterium typically found in myeloid cells. The infection is a source of severe obstetrical complications in humans and cattle and can undergo chronic evolution in a minority of pregnant women. Because C. burnetii is found in the placentas of aborted fetuses, we investigated the possibility that it could infect trophoblasts. Here, we show that C. burnetii infected and replicated in BeWo trophoblasts within phagolysosome ...[more]

PMID: 21179488

Similar Datasets

Chromatin immunoprecipitation using anti-myc 9E11 of myc-tagged GCN4-harboring yeast after heat shock treatment

Project description:heat shock of myc tagged GCN4 harboring cells- then chip-chip

2009-06-01 | E-MEXP-1679 | biostudies-arrayexpress

Chromatin immunoprecipitation using anti-myc 9E11 of myc-tagged GCN4-harboring yeast treated with DTT

Project description:DTT Treatment of myc tagged GCN4 harboring cells- then chip-chip

2009-06-10 | E-MEXP-1678 | biostudies-arrayexpress

Transcription profiling of two mesothelioma cell lines (MPP89 and REN), and of HMC-TERT non tumoral mesothelial cells

Project description:miRNA profile analysis of two mesothelioma cell lines (MPP89 and REN), and of HMC-TERT non tumoral mesothelial cells

2008-10-09 | E-MEXP-1699 | biostudies-arrayexpress

Transcription profiling of human myelogenous leukemia K562 cells expressing BASP1

Project description:We have analysed the dynamic between WT1 and BASP1 in the regulation of gene expression in myelogenous leukaemia K562 cells. Our analysis reveals that BASP1 is a significant regulator of WT1 that is recruited to WT1-binding sites and suppresses WT1-mediated transcriptional activation at several WT1 target genes. We found that WT1 and BASP1 can divert the differentiation program of K562 cells to a non-blood cell type following induction by the phorbol ester PMA. Cell lines were generated expressing the BASP1 gene from the vector pcDNA3. Array analysis was performed on 4 conditions: cells expressing BASP1 and controls, in the absence and presence of Phorbol 12-myristate 13-acetate (PMA).

2010-12-18 | E-MEXP-2573 | biostudies-arrayexpress

BIN-67 small cell ovarian carcinoma Expression array

Project description:The biology of small cell ovarian carcinoma of the hypercalcemic type (SCCOHT), which is a rare and aggressive form of ovarian cancer, is poorly understood. Tumourigenicity, in vitro growth characteristics, genetic and genomic anomalies, and sensitivity to standard and novel chemotherapeutic treatments were investigated in the unique SCCOHT cell line, BIN-67, to provide further insight in the biology of this rare type of ovarian cancer. Microarray expression analysis was performed using the GeneChip? Human Genome U133 Plus 2.0 Array (Affymetrix?, Santa Clara, CA) with total RNA from BIN-67 cells. Hybridization and scanning were performed at the McGill University and Genome Quebec Innovation Centre (Montreal, QC). Gene expression levels were determined from the scanned images using Affymetrix? Microarray Suite (MAS) version 5.0 software expression algorithm (Affymetrix?, Santa Clara, CA). Gene expression profiles were compared with Affymetrix U133 Plus 2.0 (MAS5.0) generated expression profiles (similarly normalized) from 10 human normal ovarian surface epithelial (OSE) cell brushings available in the ArrayExpress database (www.ebi.ac.uk/arrayexpress/), accession number E-GEOD-18520.

2013-09-24 | E-MEXP-3872 | biostudies-arrayexpress

calcitriol triggers axogenesis and myelination

Project description:Using pangenomic cDNA microarrays and qPCR techniques, we identified the genes regulated by calcitriol (1,25 (OH)D3, 10 nM) in dorsal root ganglia and/or Schwann cells. After 24 hours of calcitriol supplementation, we found a modified expression of many genes involved in axogenesis and myelination.

2012-04-07 | E-MEXP-3491 | biostudies-arrayexpress

MicroRNAs regulate DC maturation via M-CSFR

Project description:We identify a panel of microRNAs that are differentially expressed during both spontaneous and LPS-induced DC maturation and show the M-CSF receptor (M-CSFR) as a key target for microRNA-mediated regulation. MicroRNA-22, -34a and -155 are up-regulated in mature GM-CSF-generated DC and mediate M-CSFR mRNA and protein down-regulation.

2013-11-04 | E-MEXP-3311 | biostudies-arrayexpress

Transcription profiling of mouse naive T cells differentiated under TH1 and TH2 conditions

Project description:Naive CD4+ CD62L+ CD25- T cells were differentiated under TH1 and TH2 conditions for 7 days, restimulated with anti-CD3 and anti-CD28 for 24h and sorted for IFN-gamma (TH1) and IL-4 (TH2) production using cytokine secretion assays.

2009-06-01 | E-MEXP-2153 | biostudies-arrayexpress

Transcription profiling by array of yeast haploid Sigma1278b strains to detect Yak1-regulated genes

Project description:We determined and classified Yak1-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of different haploid MATa yeast strains of the following genotypes:YAK1 (468=YHUM0468), yak1 (1313=YHUM1313), strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 (1313Yhc) and strain YHUM1313 (yak1) carrying a high copy plasmid with YAK1 K398R (1313KDhc). All strains were grown in duplicate in YNB medium supplemented with tryptophan at 30 degrees Celsius to an optical density of 1.0 before extraction of total RNA and transcriptional profiling.

2011-08-23 | E-MEXP-2885 | biostudies-arrayexpress

Transcription profiling of human mesothelioma cells MPP-89 REN compared to mesothelial cells HMC-TERT

Project description:gene expression profile of mesothelioma cells (MPP-89; REN) compared to mesothelial cells (HMC-TERT)

2008-10-09 | E-MEXP-1701 | biostudies-arrayexpress