Transcription profiling by array of Rhizobium leguminosarum biovar viciae strain Rlv3841 grown rhizosphere of either pea, alfalfa or sugarbeet
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ABSTRACT: Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown for 7 days in the rhizosphere of either 7 day old pea, alfalfa or sugarbeet before being harvested.
Project description:Rhizobium leguminosaeum biovar viciae strain Rlv3841 was inoculated at either 10_3 CFU ot 10_8 CFU into the rhizosphere of 7 d old peas and the bacteria were harvested 7 days post inoculation. They were compared indircetly against a control of glucose ammonia grown laboratory cells.
Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown for 7 d in the pea or alfalfa or sugarbeet rhizosphere (plants all 7 d old at inoculation) and compared against bacteria grown on glucose ammonia AMS medium in the laboratory.
Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was inoculated into the rhizosphre of 7 d old peas and left for 1, 3 or 7 days before harvest and gene expression analysed.
Project description:Strain PtsP107 has a Tn5 insertion in PtsP and it was grown on glucose/ ammonia minimal medium and gene expression compared to the wild type Rhizobium leguminosarum biovar viciae strain Rlv3841 grown on the same medium.
Project description:Pea plants of 7, 14 or 21 were infected with Rhizobium leguminosarum biovar viciae strain Rlv3841 and bacterial gene expression measured 24 hours later. Expression was compared to bacteria grown in the laboratory on glucose ammonia.
Project description:Rhizobium leguminosarum bivar viciae strain 3841 was grown on AMS minimal medium with either succinate ammonia or glucose ammonia as carbon and nitrogen sources. Gene expression was compared between the two crabon sources. Total RNA was amplified using Genispheres SenseAmp kit.
Project description:Wildtype bacteroids of Rhizobium leguminosarum biovar viciae 3841 were compared to a bacA mutant. In both cases bacteroids were harvested from 7 day old nodules from pea plants.
Project description:Rhizobium leguminosarum biovar viciae strain Rlv3841 was grown on a variety of compounds suspected of being in the rhizosphere and gene induction was compared to cells grown on either pyruvate NH4 or glucose NH4.
Project description:Rhizobium leguminosarum biovar viciae strain RU2386 was grown overnight on glc asp and the next day it was resuspended in glucose glutamate. Gene expression before resuspension was compared to the 2 h resuspended sample.
Project description:Rlv Rhizobium leguminosarum Rlv3841 was grown overnight on glucose (10 mM) aspartate (10 mM) and then washed and resuspended in glucose (10 mM) glutamate (10 mM) after 2 h transcription was measured