Project description:Both wild-type and mutant A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in GM plates for 3 weeks under a 16 h light/8 h dark regimen (40 ± 10 umol photons/m2/s) at 22°C. Dehydration treatment: The 3-week-old plants were grown for 2 h without watering.
Project description:We compared the expression profiles of amiG7 mutant with those of the wild type plants using an Agilent Arabidopsis 3 Oligo Microarray.
Project description:Both wild-type and SPY-3 OX A. thaliana plants (Arabidopsis thaliana L. ecotype-Columbia) were grown in GM plates for 3 weeks under a 16 h light/8 h dark regimen (40 M-1 10 umol photons/m2/s) at 22M-0C. Dehydration treatment: The 3-week-old plants were grown for 2 h without watering.
Project description:Three-week-old DEX-AZF1, DEX-AZF2, and vector control plants were treated 10 µM DEX solution for 24 h. Two-week-old ProAZF2:AZF2 and vector control plants were treated with water as control or 200 mM NaCl for 5 h.
Project description:Arabidopsis thaliana (L.) Heynh. ecotype Columbia was used in this study. Seeds were sterilized and stratified at 4 C for 2 days in the dark, sown, and grown on germination media (GM) agar plates in a growth chamber with 16 hours of fluorescent light at 40M-110 ?mol m-2 sec-1, 22 C, and 70% relative humidity.<br><br> The T-DNA insertion line for GRF7 was obtained from the ABRC (Stock No. CS878963;? Sessions et al., 2002). The T-DNA insertion was confirmed by amplifying the LB-flanking region in genomic DNA of individual candidate lines.
Project description:Expression profiles of the wild-type Arabidopsis (Col) and srk2dei triple mutant plants in response to ABA, NaCl, and dehydration treatments.<br><br>
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates.
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with or without 50 µM ABA for 6 h.