Methylation profiling by array of mouse wild type and C9a knockout embyronic stem cells uisng antibodies against 5mC

ABSTRACT: Promoter DNA methylation in mouse ES cells (wild type and G9a knock-out) was assessed using an antibody against 5mC on fragmented genomic DNA and subsequent purification of the methylated DNA by affinity through a column containing the methyl-GpG binding domain of MeCP2 (MAP: methylation affinity purification). Purified DNA, enriched for methylated DNA was hybridised to promoter arrays in pairs input/MAP.

ORGANISM(S): Mus musculus

SUBMITTER: Jose de las Heras 

PROVIDER: E-MEXP-2872 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress