Transcription profiling of C. elegans small RNA cloning and array methods for reproducibility and quantitation

ABSTRACT: This experiment was for the purpose of testing our small RNA cloning and array methods for reproducibility and quantitation (see Axtell and Bartel). This experiment consists of four hybridizations of C. elegans small RNA. Hybridizations test a Cy3-labeled biological sample against a constant, synthetic reference sample (Cy5). Two technical replicates are used; two indepedent clonings and labelings of small RNAs from a C. elegans wild-type, mixed stage RNA sample, and two from glp-4(bn2) worms. This experiment tested the technical reproducibility of the array at two different hyb temperatures, 50C and 57C. Biological samples were labeled Cy3, while a constant, synthetic reference sample was labeled Cy5.

ORGANISM(S): synthetic DNA

SUBMITTER: David Bartel 

PROVIDER: E-MEXP-291 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress