Arabidopsis microRNA Organ Map

ABSTRACT: This experiment was designed to determine the presence and levels of microRNAs and other select small RNAs in the major organs of wild-type (Col-0) Arabidopsis thaliana grown under standard conditions.



To obtain a global overview of miRNA levels in wild-type Arabidopsis, a tissue expression map was generated. Two samples of total RNA from siliques, stems, cauline leaves, roots, short-day seedlings, long-day seedlings, and rosette leaves as well as four samples from inflorescences were obtained. All duplicate samples were harvested from independent crops of plants grown under the same conditions, and thus represent biologcial replicates. Hybridizations were always comparing a given sample against a common, synthetic reference set. 225 reference DNA oligos comprising the sense strand of each RNA represented on the array flanked by constant 5' and 3' sequences representing the adapters used in RNA cloning (Axtell and Bartel). Amplification and Cy5 end-labeling of this synthetic set of oligos provides a constant reference signal that allows comparison of different Cy3-labeled biological samples.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Michael Axtell 

PROVIDER: E-MEXP-292 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress