Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

VM-zfIFNg

ABSTRACT: Transcriptomic profile of zebrafish larvae microinjected with IFNg1 expression construct

ORGANISM(S): Danio rerio

SUBMITTER: Victoriano Mulero

PROVIDER: E-MEXP-2948 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Evolutionary conserved pro-inflammatory and antigen presentation functions of zebrafish IFNγ revealed by transcriptomic and functional analysis.

López-Muñoz Azucena A Sepulcre María P MP Roca Francisco J FJ Figueras Antonio A Meseguer José J Mulero Victoriano V

Molecular immunology 20110226 9-10

In mammals, IFNγ is the only type II IFN member, whereas most bony fish species have two IFNγ genes, namely IFNγ1 and IFNγ2. We report that both zebrafish IFNγ genes were unable to protect zebrafish larvae against viral infection, despite the fact that they moderately induced the expression of antiviral genes, strongly induced pro-inflammatory and antigen processing and presentation genes, and increased neutrophil numbers. Although both zebrafish IFNγs induced a similar set of immune genes, IFNγ ...[more]

PMID: 21354627

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Project description:Phenotypic plasticity, the ability of one genotype to express different phenotypes in response to changing environmental conditions, is one of the most common phenomena characterising the living world and is not only relevant for the ecology but also for the evolution of species. Daphnia, the waterflea, is a textbook example for predator induced phenotypic plastic defences including changes in life-history, behaviour and morphology. However, the analysis of molecular mechanisms underlying these inducible defences is still in its early stages. We exposed Daphnia magna to chemical cues of the predator Triops cancriformis to identify key processes underlying plastic defensive trait formation. D. magna is known to develop an array of morphological changes in the presence of T. cancriformis including changes of carapace morphology and cuticle hardening. To get a more comprehensive idea of this phenomenon, we studied four different genotypes originating from habitats with different predation history, reaching from predator-free to temporary habitats containing T. cancriformis. We analysed the morphologies as well as proteomes of predator-exposed and control animals. Three genotypes showed morphological changes when the predator was present. Using a high-throughput proteomics approach, we found 294 proteins which were significantly altered in their abundance after predator exposure in a general or genotype dependant manner. Proteins connected to genotype dependant responses were related to the cuticle, protein synthesis and calcium binding whereas the yolk protein vitellogenin increased in abundance in all genotypes, indicating their involvement in a more general response. Furthermore, genotype dependant responses at the proteome level correlated well with local adaptation to Triops predation. Altogether, our study provides new insights concerning genotype dependant and general molecular processes involved in predator-induced phenotypic plasticity in D. magna.

Dataset Information

VM-zfIFNg

Publications

Evolutionary conserved pro-inflammatory and antigen presentation functions of zebrafish IFNγ revealed by transcriptomic and functional analysis.

Similar Datasets

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