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Transcription profiling of mouse primary neural stem cells (neurospheres) originating from separate isolations (individuals), different passages and parallel cultures

ABSTRACT: Investigation of transcriptome variability in neurospheres originating from separate isolations (individuals), different passages and parallel cultures. As a control neurospheres were also compared to neurospheres induced to differentiate by adding serum and plating onto solid support.

ORGANISM(S): Mus musculus

SUBMITTER: Valtteri Wirta 

PROVIDER: E-MEXP-297 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis in primary neural stem cells using a tag cDNA amplification method.

Sievertzon Maria M   Wirta Valtteri V   Mercer Alex A   Meletis Konstantinos K   Erlandsson Rikard R   Wikström Lilian L   Frisén Jonas J   Lundeberg Joakim J  

BMC neuroscience 20050415

<h4>Background</h4>Neural stem cells (NSCs) can be isolated from the adult mammalian brain and expanded in culture, in the form of cellular aggregates called neurospheres. Neurospheres provide an in vitro model for studying NSC behaviour and give information on the factors and mechanisms that govern their proliferation and differentiation. They are also a promising source for cell replacement therapies of the central nervous system. Neurospheres are complex structures consisting of several cell  ...[more]

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