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Dataset Information

Spheres culture from lung adenocarcinoma pleural effusions

ABSTRACT: Briefly, we analyzed gene expression profiles in adherent cultures compared to sphere cultures from malignant pleural effusions (MPEs). MPEs could represent an opportunity to culture a wide variety of cancer cells from different donors. In this study, we set up culture conditions for cancer cells deriving from MPEs of several patients affected by the most frequent form of lung cancer, namely the subset of non small cell lung cancers (NSCLC) classified as Lung Adenocarcinomas, to show that MPEs are an excellent source of tumor initiating cells for the study of lung adenocarcinoma.

ORGANISM(S): Homo sapiens

SUBMITTER: Pietro Zoppoli

PROVIDER: E-MEXP-3025 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Spheres derived from lung adenocarcinoma pleural effusions: molecular characterization and tumor engraftment.

Mancini Rita R Giarnieri Enrico E De Vitis Claudia C Malanga Donatella D Roscilli Giuseppe G Noto Alessia A Marra Emanuele E Laudanna Carmelo C Zoppoli Pietro P De Luca Pasquale P Affuso Andrea A Ruco Luigi L Di Napoli Arianna A Mesiti Giuseppe G Aurisicchio Luigi L Ricci Alberto A Mariotta Salvatore S Pisani Lara L Andreetti Claudio C Viglietto Giuseppe G Rendina Erino A EA Giovagnoli Maria Rosaria MR Ciliberto Gennaro G

PloS one 20110718 7

Malignant pleural effusions (MPEs) could represent an excellent source to culture a wide variety of cancer cells from different donors. In this study, we set up culture conditions for cancer cells deriving from MPEs of several patients affected by the most frequent form of lung cancer, namely the subset of non small cell lung cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which account for approximately 40% of lung cancer cases. AdenoCa malignant pleural effusions gave rise to in v ...[more]

PMID: 21789168

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Project description:The traditional method for studying cancer in vitro is to grow immortalized cancer cells in two-dimensional (2D) monolayers on plastic. However, many cellular features are impaired in these unnatural conditions and big alterations in gene expression in comparison to tumors have been reported. Three-dimensional (3D) cell culture models have become increasingly popular and are suggested to be better models than 2D monolayers due to improved cell-to-cell contacts and structures that resemble in vivo architecture. The aim of this study was to develop a simple high-throughput 3D drug screening method and to compare drug responses in JIMT1 breast cancer cells when grown in 2D, in polyHEMA coated anchorage independent 3D models and in Matrigel on-top 3D cell culture models. We screened 102 compounds with multiple concentrations and biological replicates for their effects on cell proliferation. The cells were either treated immediately upon plating or they were allowed to grow in 3D for four days prior to the drug treatment. Big variations in drug responses were observed between the models indicating that comparisons of culture model influenced drug sensitivities cannot be made based on effects of a single drug. However, we show with the 63 most prominent drugs that, in general, JIMT1 cells grown on Matrigel were significantly more sensitive to drugs than cells grown in 2D cultures, while responses of cells grown in polyHEMA resembled those of 2D. Furthermore, comparison of gene expression profiles of the cell culture models to xenograft tumors indicated that cells cultured in Matrigel and as xenografts most closely resembled each other. In this study we also suggest that 3D cultures can provide a platform for systematic experimentation of larger compound collections in a high-throughput mode and be used as alternatives for traditional 2D screens towards better comparability to in vivo state. Gene expression analysis of JIMT1 breast cancer cells cultured as xenografts for 43 days, in two dimensional cultures for seven days (2D7d), in polyHEMA three dimensional cell culture models for four and seven days (PH7d and PH7d), and in Matrigel three dimensional cultures for four and seven days (MG4d and MG7d). Two biological replicates was included for each sample.

Dataset Information

Spheres culture from lung adenocarcinoma pleural effusions

Publications

Spheres derived from lung adenocarcinoma pleural effusions: molecular characterization and tumor engraftment.

Similar Datasets

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