Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transfer of 5d old Col-0 and WEE1 KO seedlings to medium supplemented with 2mM HU for 0h-5h-24h

ABSTRACT: Col-0 and WEE1KO (wee1-1 and wee1-2) were germinated on control medium on a nylon mesh and transferred 5 days after germination to medium supplemented with 2 mM HU. Samples were harvested at three different time points after transfer: 0 h, 5 h, and 24 h. All sampling points were performed in four independent experiments for Col-0, two independent experiments for wee1-1 and 2 independent experiments for wee1-2. For each time point in each experiment ﾱ50 root tips were collected and frozen in liquid nitrogen. RNA was extracted from root tissue with TriZol reagent (Invitrogen) and purified with RNneasy kit (Qiagen). The RNA of two independent experiments for Col-0 and WEE1KO were subsequently pooled and used for
microarray analysis.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Toon Cools 

PROVIDER: E-MEXP-3053 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

The Arabidopsis thaliana checkpoint kinase WEE1 protects against premature vascular differentiation during replication stress.

Cools Toon T   Iantcheva Anelia A   Weimer Annika K AK   Boens Shannah S   Takahashi Naoki N   Maes Sara S   Van den Daele Hilde H   Van Isterdael Gert G   Schnittger Arp A   De Veylder Lieven L  

The Plant cell 20110415 4

A sessile lifestyle forces plants to respond promptly to factors that affect their genomic integrity. Therefore, plants have developed checkpoint mechanisms to arrest cell cycle progression upon the occurrence of DNA stress, allowing the DNA to be repaired before onset of division. Previously, the WEE1 kinase had been demonstrated to be essential for delaying progression through the cell cycle in the presence of replication-inhibitory drugs, such as hydroxyurea. To understand the severe growth a  ...[more]