Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bordetella bronchiseptica RB50 iron limitation

ABSTRACT: B. bronchiseptica RB50 was grown in medium either lacking or containing iron (ferrous sulfate). At various time points, samples were taken for gene expression analysis.

ORGANISM(S): Bordetella bronchiseptica

SUBMITTER: Craig Cummings

PROVIDER: E-MEXP-3060 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Bordetella bronchiseptica RB50 iron limitation 2

Project description:Bordetella bronchiseptica RB50 was shifted from iron replete to either iron depleted or iron replete media, and samples were taken post shift for transcriptional profiling

2011-09-08 | E-MEXP-3076 | biostudies-arrayexpress

Bordetella pertussis Tohama I iron limitation 1

Project description:B. pertussis Tohama I was grown in iron-depleted or iron-replete media and sampled at several time points to assess global gene expression

2011-09-08 | E-MEXP-3263 | biostudies-arrayexpress

Comparative genomic hybridization of Bordetella bronchiseptica strains RB50 and 1289

Project description:B. bronchiseptica strains RB50 and 1289 strains were grown in SS broth at 37°C with shaking overnight and genomic DNA was isolated from bacterial cultures using a DNA extraction kit (Qiagen, Valencia, CA) and digested with DpnII. For each labeling reaction, 2 ug of digested genomic DNA was randomly primed using Cy-5 and Cy-3 dye-labeled nucleotides, with BioPrime DNA labeling kits (Invitrogen, Carlsbad, CA) and the two differentially labeled reactions to be compared were combined and hybridized to a B. bronchiseptica RB50 specific long-oligonucleotide microarray.

2008-12-31 | E-MEXP-1737 | biostudies-arrayexpress

Shotgun proteomic analysis of Bordetella pertussis response to iron availability

Project description:B. pertussis Tohama I was cultivated in iron-depleted or iron-repleted medium in order to monitor proteins which are influence by iron supply. More than 200 proteins displayed increased or decreased levels. Proteins involved in iron acquisition, biofilm production and oxidative stress response were increased during iron starvation. Limited iron supply caused on the other hand decreased levels of virulence factors regulated by the BvgAS system.

2015-03-16 | PXD001280 | Pride

Hfq is involved in global protein expression and stress response in Bordetella pertussis

Project description:In this study, we used gel-free nanoLC-MS/MS-based proteomics to compare the protein profile of B. pertussis wild type and an isogenic hfq defective mutant strain under control an iron limited conditions. We found that Hfq affects the abundance of 302 proteins, which represents 8% of the total B. pertussis coding sequence. The absence of Hfq induced changes in the abundance of proteins involved in metabolic pathways, stress response and virulence. Hfq was also found involved in B. pertussis adaptation to iron starvation, one of the main stresses this pathogen faces inside the host. Altogether, these results indicate that B. pertussis Hfq is involved in bacterial physiological processes as well as bacterial pathogenesis.

2020-02-18 | PXD011753 | Pride

T44625-cgh

Project description:comparative genomic hybridization (CGH) analysis was perfromed in order to identify coding regions present in strain T44625 that are either absent or contain a high degree of sequence divergence compared to the RB50 reference strain.

2013-12-01 | E-MEXP-3968 | biostudies-arrayexpress

T44625

Project description:Whole genome transcriptome analysis was performed to determine which genes were differentially expressed between strains T44625 and RB50

2013-12-01 | E-MEXP-3967 | biostudies-arrayexpress

Transcription profiling of Bacillus anthracis Sterne during iron starvation

Project description:Effects of iron starvation on the transcriptional profile of Bacillus anthracis

2010-07-22 | E-MEXP-2272 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica virulent swine isolate KM22, wild type and fhaB and prn deletion mutants

Project description:B. bronchiseptica strain KM22, a virulent swine isolate, and strains TN27 and TN28, fhaB and prn deletion mutants of KM22, respectively, were cultured in Stainer-Scholte broth and grown at 37C with shaking at 275 rpm until an OD600 of 0.8 was reached. RNA extracted from KM22 was then compared to RNA extracted from TN27 and TN28.

2008-09-20 | E-MEXP-1558 | biostudies-arrayexpress

Transcription profiling of three Bordetella bronchiseptica strains at three distinct phases of growth: mid-log phase, late-log phase, and late-stationary phase

Project description:Microarray analysis was performed to determine if the growth-dependent gene expression changes occurred on a global scale for B. bronchiseptica strains RB50, RB53, and RB54. For this analysis cDNA representing three distinct phases of growth: mid-log phase (15H), late-log phase (24H), and late-stationary phase (48H) were directly compared.

2009-07-01 | E-MEXP-2015 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data