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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of wheat GF- B1118-8-4 vs B1355-4-2 transgenic lines endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination ?dpg)

ABSTRACT: Transgenic cadenza lines express five HMW subunits encoded by:Glu-B1 (14, 15) and Glu D1 (5, 10) and transgene Glu-A1 (Ax1). Transcriptomes of the wheat line B1118-8-4(6) (produced by co transformation with two plasmids: one carried the transgene HMW-GS 1Ax1and other the bar and gus gene sequences) and wheat line B1355-4-2(18) (generated by co transformation with the clean fragments of the HMW-GS 1Ax1 transgene and the bar gene sequence) were compared. Transcriptomes analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg). Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue /developmental stage selected). Hybridisations were performed in reverse dye labelling

ORGANISM(S): Triticum aestivum

SUBMITTER: Maria Baudo

PROVIDER: E-MEXP-308 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of wheat L88-18 line vs CTRL_L8831endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg)

Project description:The high molecular weight (HMW) subunits of wheat glutenin are synthesised only in the starchy endosperm tissue of the developing wheat grain. The transcriptomes of the lines L88-18 and L88-31 (Lawrence, G.J., Macritchie, F. & Wrigley, C.W. Dough and baking quality of wheat lines in glutenin subunits controlled by Glu-A1, Glu-B1 and Glu-D1 loci. J. Cereal. Sci. 7,109-112 (1988)) coming from the same cross were compared. Transcriptomes analysis was performed in endosperm tissue (14 and 28 days post anthesis-dpa) and in leaf tissue (8 days post germination dpg). Each of the transcriptome comparisons was performed using three biological replicates (i.e. per line/tissue /developmental stage selected). Hybridisations were performed in reverse dye labelling.

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