Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of AtHsp70-14 and AtHsp70-15 deficient Arabidopsis thaliana plants

ABSTRACT: AtHsp70-14 and -15 (At1g79930 and At1g79920) are two closely related isoforms of the Hsp110/SSE subfamily of the HSP70 chaperones in Arabidopsis thaliana. By analysing T-DNA insertion and amiRNA-AtHsp70-14/15 lines, we could demonstrate an essential function of AtHsp70-15 for plant growth. AtHsp70-15 deficient plants are reduced in size and stomata opening is impaired which results in accelerated wilting of detached leaves. Hsp70-15 deficient plants are also more tolerant to TuMV infection but more sensitive to heat treatment. Therefore comparative transcriptome analysis of amiRNA-AtHsp70-14/15, hsp70-14 knockout, hsp70-15 knockout and wild type plants was performed with leaf samples of 6 week old plants grown under ambient conditions. The analysis of differentially regulated genes compared to the wild type Col-0 plants exhibited a constitutive heat stress response / cytosolic protein response of Hsp70-15 deficient plants.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Isabel Jungkunz

PROVIDER: E-MEXP-3082 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV.

Jungkunz Isabel I Link Katrin K Vogel Florian F Voll Lars M LM Sonnewald Sophia S Sonnewald Uwe U

The Plant journal : for cell and molecular biology 20110428 6

Arabidopsis thaliana contains 18 genes encoding Hsp70s. This heat shock protein superfamily is divided into two sub-families: DnaK and Hsp110/SSE. In order to functionally characterize members of the Hsp70 superfamily, loss-of-function mutants with reduced cytosolic Hsp70 expression were studied. AtHsp70-1 and AtHsp70-2 are constitutively expressed and represent the major cytosolic Hsp70 isoforms under ambient conditions. Analysis of single and double mutants did not reveal any difference compar ...[more]

PMID: 21418353

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Dataset Information

Transcription profiling of AtHsp70-14 and AtHsp70-15 deficient Arabidopsis thaliana plants

Publications

AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV.

Similar Datasets

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