Unknown,Transcriptomics,Genomics,Proteomics

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MiRNA signature for cis-platinum resistant ovarian cancer cells A2780

ABSTRACT: We have done the analysis of cis-platinum susceptible and resistant ovarian cancer cell lines (A2780). After having passed sample QC on the Bioanalyser2100 and RNA measurement on the Nanodrop instrument, the samples were
labelled using the miRCURYM-^Y Hy3M-^Y/Hy5M-^Y power labeling kit and hybridized on the miRCURYM-^Y
LNA Array (v.11.0). The samples were hybridized on a hybridization station following the scheme
you outlined in the sample submission.
Analysis of the scanned slides showed that the labeling was successful as all capture probes for
the control spike-in oligo nucleotides produced signals in the expected range. The quantified signals
(background correction) were normalized using the global Lowess (LOcally WEighted Scatterplot
Smoothing) regression algorithm, which we have found produces the best within-slide normalization
to minimize the intensity-dependent differences between the dyes. The positive effect of this
normalization is illustrated in the MA-plots for each slide (Project_Summary_Report.xls, sheet
SlideX).
In conclusion, the miRNA expression profiling has successfully been completed .
The miRNA profiling identified a subset (11) of the total number of miRNAs analyzed by the
miRCURYM-^Y array that are differentially expressed between the two different sample groups.

ORGANISM(S): Homo sapiens

SUBMITTER: Siva Kumar Panguluri

PROVIDER: E-MEXP-3141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MiRNA signature for cis-platinum resistant ovarian cancer cells A2780

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