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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-chip by array of H2BK123ub, H3K4me3, H3K36me3, H3K79me3, and H3K79me2 using Affymetrix tiling arrays.

ABSTRACT: Genome-wide mapping of H2BK123ub, H3K4me3, H3K36me3, H3K79me3, and H3K79me2 using Affymetrix tiling arrays.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Julia Schulze

PROVIDER: E-MEXP-3217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Splitting the task: Ubp8 and Ubp10 deubiquitinate different cellular pools of H2BK123.

Schulze Julia M JM Hentrich Thomas T Nakanishi Shima S Gupta Arvind A Emberly Eldon E Shilatifard Ali A Kobor Michael S MS

Genes & development 20111101 21

Monoubiquitination of H2BK123 (H2BK123ub), catalyzed by Rad6/Bre1, is a transient histone modification with roles in transcription and is essential for establishing H3K4 and H3K79 trimethylations (H3K4me3 and H3K79me3). Here, we investigated the chromatin network around H2BK123ub by examining its localization and co-occurrence with its dependent marks as well as the transcription elongation mark H3K36me3 across the genome of Saccharomyces cerevisiae. In yeast, H2BK123ub is removed by the deubiqu ...[more]

PMID: 22056669

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