Transcription profiling of amino-acid starved yeast cells to monitor the transcript level and also the translation status for each RNA

ABSTRACT: Global restriction of protein synthesis is a hallmark of cellular stress. Using amino-acid starvation, we monitor the transcript level and also the translation status for each RNA using cycloheximide to freeze elongating ribosomes. Polyribosome fractionation of cell extracts was used to seperate highly translated and poorly translated mRNAs that were then seperately analysed.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ioulia Smirnova 

PROVIDER: E-MEXP-323 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress