Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development

ABSTRACT: We were testing the expression differences of seuss, aintegumenta, and double mutants in stage 8-10 dissected carpels from Arabidopsis thaliana.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: April Wynn

PROVIDER: E-MEXP-3293 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Wynn April N AN Rueschhoff Elizabeth E EE Franks Robert G RG

PloS one 20111021 10

In flowering plants the gynoecium is the female reproductive structure. In Arabidopsis thaliana ovules initiate within the developing gynoecium from meristematic tissue located along the margins of the floral carpels. When fertilized the ovules will develop into seeds. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). The synergistic loss of ovule initiation observed in the seu ant doub ...[more]

PMID: 22031826

Similar Datasets

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development

Project description:We are interested in examining the transcriptional hierarchies that are required for proper formation and function of the carpel margin meristem in Arabidopsis thaliana. The carpel margin meristem (CMM) gives rise to the ovule primorida (which begin to form around flower stage 8). We know that the loss of the seuss-3 (seu-3) and aintegumenta-1(ant-1) separately leads to a mild disruption of ovule formation, while the loss of both causes a complete loss of ovule formation. In this study we wanted to examine expression levels changed between each single mutant and the double mutant. We dissected out stage 8-10 carpel of each of the four genotypes (seu-3, ant-1, Col-0, seu-3 ant-1) pooling 15-25 carpels into one biological replicate. Then using 16 ATH1 arrays we hybridized, amplified and labeled cDNA to 16 arrays (4 replicates for each genotype). We then analyzed our data to reveal transcripts that went down in the double compared to either single mutant, and transcripts that went down more than additively in the double mutant compared to added level of the decrease of the single mutant levels. 16 arrays: 4 seuss-3, 4 aintegumenta-1, 4 Col-0, 4 seuss-3 aintegumenta-1. Each biological replicate was a sample of 15-25 pooled stage 8-10 carpels manually dissected by hand.

2011-07-31 | E-GEOD-30492 | biostudies-arrayexpress

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana

2011-08-01 | GSE30492 | GEO

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana.

Project description: Not available

| S-EPMC3198736 | biostudies-literature

Transcription profiling of pistils from Arabidopsis wild type, ant-4 mutant and ino-1mutant plants at different developmental stages

Project description:This experiment was designed to identify genes expressed preferentially in the two integuments of the Arabidopsis ovule. Pistils from wild type and two ovule mutants were compared against each aintegumenta-4 (ant-4) which lacks both integuments and inner no outer (ino-1) which lacks the outer integument. Genes that are highly expressed only in the integuments were expected to be reduced in expression in the mutants, as compared with wild type. Pistils containing ovules through all stages of ovule development prior to pollination were pooled for one experiment (FULL arrays), and for two separate experiments, a set of early differentiation stages (EARLY arrays) and a set of later differentiation stages (LATE ARRAYS) were pooled. Wild type and mutant lines are in the ecotype Landsberg erecta.

2009-06-01 | E-MEXP-1920 | biostudies-arrayexpress

The CUC1 and CUC2 genes promote carpel margin meristem formation during Arabidopsis gynoecium development.

Project description: Not available

| S-EPMC4012194 | biostudies-literature

Transcription profiling by array of mouse bone marrow proB cells treated with IL-7 or CpG

Project description:Culture of proB cells with IL7 in the presence or absence of CpG

2010-02-27 | E-MEXP-2595 | biostudies-arrayexpress

Transcription profiling of yeast sum1 delta strain at three time points after the start of meiosis

Project description:Expression profiling of sum1 delta strain at three time points after the start of meiosis

2010-04-20 | E-MEXP-2154 | biostudies-arrayexpress

Transcription profiling of yeast during meiosis in a strain with Ndt80 induced with estradiol

Project description:Expression profile of meiosis in strain with Ndt80 inducible by addition of Estradiol (as in Carlile and Amon 2008), in three time points from addition of Estradiol.

2010-04-20 | E-MEXP-2155 | biostudies-arrayexpress

Transcription profiling of yeast W303 cells at multiple time points during meiosis

Project description:expression profiling of meiotic W303 cells in duplicate- multiple time points

2009-07-28 | E-MEXP-1781 | biostudies-arrayexpress

Transcription profiling of yeast pdr mutants

Project description:PDR mutants , strain YYA100 (pdr1?::KanMx6, pdr3?::His3Mx6) from Mol Biol Cell. 2007 December; 18(12): 49324944, was grown to OD 1-1.5 in YEPD and hybridyzed to an expression array in one channel with a wild-type strain reference RNA on the other channel

2009-06-17 | E-MEXP-2150 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data