Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Aspergillus fumigatus Af293 after temperature shifting

ABSTRACT: Experimental factor: Time after the temperature shift from 30C to 37C or 30C to 48C. Conidia (5x106 /ml) from Aspergillus fumigatus Af293 were incubated in the CM medium for germination (~ 17 hrs) at 30C. The culture was transferred to a water bath of 37C or 48C for continued growth. Samples were taken after the defined time (i.e. 0, 15, 30, 60, 120, and 180 min.).

ORGANISM(S): Aspergillus fumigatus

SUBMITTER: H. Stanley Kim

PROVIDER: E-MEXP-332 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.

Nierman William C WC Pain Arnab A Anderson Michael J MJ Wortman Jennifer R JR Kim H Stanley HS Arroyo Javier J Berriman Matthew M Abe Keietsu K Archer David B DB Bermejo Clara C Bennett Joan J Bowyer Paul P Chen Dan D Collins Matthew M Coulsen Richard R Davies Robert R Dyer Paul S PS Farman Mark M Fedorova Nadia N Fedorova Natalie N Feldblyum Tamara V TV Fischer Reinhard R Fosker Nigel N Fraser Audrey A García Jose L JL García Maria J MJ Goble Arlette A Goldman Gustavo H GH Gomi Katsuya K Griffith-Jones Sam S Gwilliam Ryan R Haas Brian B Haas Hubertus H Harris David D Horiuchi H H Huang Jiaqi J Humphray Sean S Jiménez Javier J Keller Nancy N Khouri Hoda H Kitamoto Katsuhiko K Kobayashi Tetsuo T Konzack Sven S Kulkarni Resham R Kumagai Toshitaka T Lafon Anne A Latgé Jean-Paul JP Li Weixi W Lord Angela A Lu Charles C Majoros William H WH May Gregory S GS Miller Bruce L BL Mohamoud Yasmin Y Molina Maria M Monod Michel M Mouyna Isabelle I Mulligan Stephanie S Murphy Lee L O'Neil Susan S Paulsen Ian I Peñalva Miguel A MA Pertea Mihaela M Price Claire C Pritchard Bethan L BL Quail Michael A MA Rabbinowitsch Ester E Rawlins Neil N Rajandream Marie-Adele MA Reichard Utz U Renauld Hubert H Robson Geoffrey D GD Rodriguez de Córdoba Santiago S Rodríguez-Peña Jose M JM Ronning Catherine M CM Rutter Simon S Salzberg Steven L SL Sanchez Miguel M Sánchez-Ferrero Juan C JC Saunders David D Seeger Kathy K Squares Rob R Squares Steven S Takeuchi Michio M Tekaia Fredj F Turner Geoffrey G Vazquez de Aldana Carlos R CR Weidman Janice J White Owen O Woodward John J Yu Jae-Hyuk JH Fraser Claire C Galagan James E JE Asai Kiyoshi K Machida Masayuki M Hall Neil N Barrell Bart B Denning David W DW

Nature 20051201 7071

Aspergillus fumigatus is exceptional among microorganisms in being both a primary and opportunistic pathogen as well as a major allergen. Its conidia production is prolific, and so human respiratory tract exposure is almost constant. A. fumigatus is isolated from human habitats and vegetable compost heaps. In immunocompromised individuals, the incidence of invasive infection can be as high as 50% and the mortality rate is often about 50% (ref. 2). The interaction of A. fumigatus and other airbor ...[more]

PMID: 16372009

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Project description:Wounding due to mechanical injury or insect feeding causes a wide array of damage to the cell including cell disruption, desiccation, metabolite oxidation, and disruption of primary metabolism. In response, plants regulate a variety of genes and metabolic pathways to cope with injury. Tomato (Solanum lycopersicum) is a model for wound signaling but few studies have examined the comprehensive gene expression profiles in response to injury. A cross-species microarray approach using the TIGR potato 10-K cDNA array was analyzed for large-scale temporal (early and late) and spatial (locally and systemically) responses to mechanical wounding in tomato leaves. These analyses demonstrated that tomato regulates many primary and secondary metabolic pathways and this regulation is dependent on both timing and location. To determine if LAP-A, a known modulator of wound signaling, influences gene expression beyond the core of late wound-response genes, changes in RNAs from healthy and wounded Leucine aminopeptidase A-silenced (LapA-SI) and wild-type (WT) leaves were examined. While most of the changes in gene expression after wounding in LapA-SI leaves were similar to WT, overall responses were delayed in the LapA-SI leaves. Moreover, two pathogenesis-related 1 (PR-1c and PR-1a2) and two dehydrin (TAS14 and Dhn3) genes were negatively regulated by LAP-A. Collectively, this study has shown that tomato wound responses are complex and that LAP-A's role in modulation of wound responses extends beyond the well-described late-wound gene core. A reference design hybridization strategy was used with WT (0-hr) being the reference RNA. The reference RNA was a pool from five WT 0-hr RNAs. RNAs from wounded or unwounded leaves labeled with Cy3 and the reference RNA labeled with Cy5 were co-hybridized to the potato cDNA array. Three dye bias experiments were performed in which WT 0-hr RNAs were labeled with Cy3 and co-hybridized with the Cy5-labeled reference RNA (WT 0-hr RNAs from a different pool). After normalization, no dye bias was detected on these experiments (p-value<0.05).

Dataset Information

Transcription profiling of Aspergillus fumigatus Af293 after temperature shifting

Publications

Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.

Similar Datasets

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