Transcriptional profiling by array of human adipose-derived stem cells to study the influence of cell-secreted ECM on the osteogenic differentiation process.

ABSTRACT: The aim of this study is to characterize how the extracellular matrix secreted by adipose-derived stem cells (ADSC) during osteogenesis affects the differentiation process. Specifically, ADSC undergo osteogenesis by following similar maturational phases as bone marrow-derived stem cells. However, it is unclear how the differentiation process is the same and how it differs. We first focused on ADSC behavior by analyzing whole transcriptome changes in response to osteogenic media supplements added into the tissue culture medium. We then developed osteogenic differentiation expression profiles for the ADSC and identify key genes and pathways that serve as an osteogenesis signature. This expression signature acted as a template for comparison of how extracellular matrix (ECM) affected ADSC

ORGANISM(S): Homo sapiens

SUBMITTER: Heather Coan 

PROVIDER: E-MEXP-3340 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress