ABSTRACT: Pools of 3 E14 eda null half skin pairs were cultured for 90 minutes or 4 hours in hanging drops of medium containing 2ug/mL of Fc-Eda-A1 (treated halves) or the equivalent amount of solvent (control halves). Biological triplicates for each condition were performed.
Project description:Effects of furanone on global gene expression. CD-1mice were treated with compond A B or C for 21 days. The compoud was administered orally to each mouse using a gastric sonde.The researcher was not previously informed about wich compound was the control, this to ensure impartiality during the experiment and the outcoume of the results.
Project description:An exponentially growing C. albicans SC5314 culture in SD medium at 30 C was split into two flasks, one exposed to MIC90 concentration of compound 2 (6.25 ug./ml-1)mannich ketones, the other to the same volume of water. Samples were collected after 30 and 60 min for transcript profiling.
Project description:We found that the antibiotic colistin acts synergistically with antifungals of the echinocandin class (e.g. aminocandin) on C. albicans cells. In order to elucidate the mode of action of colistin in fungi we performed microarray analysis of samples treated with only aminocandin (0.00125µg/ml) or treated with aminocandin (0.00125µg/ml) and colistin (5µg/ml). We compared: (A). untreated cells to cells treated with aminocandin only; (B). cells treated with aminocandin to cells treated with aminocandin and colistin. By comparing those datasets it should be possible to identify genes differentially expressed in response to aminocandin and in response to both drugs. And subsequently to be able to interpret where in the cell colistin acts. (See related experiment in ArrayExpress: E-MEXP-3438)
Project description:chromatin immunoprecipitations were performed in mouse haematopoietic and endothelial cell lines using an anti-H3 trimethyl lysine 4 antibody
Project description:Human pancreatic islets were isolated from pancreas of deceased donors by Ricordi's procedure and cultured in CMRL 1066 medium additioned with human albumin. EVs were isolated from conditioned medium derived from islet culture after isolation. Once isolated, RNA of islets and islet-derived EVs was extracted and analyzed for microRNA expression within 48 hours after isolation.
Project description:Wild Type and OsTZF1-OX plants were grown in soil in isolation green house for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (1000 umol photons/m2/s) at 28C (day) and 25C (night).
Project description:Most sRNAs control the expression of target genes by interacting with their mRNA. The fvr1 (Francisella virulence RNA 1) gene is found in the IGR between FTL_0777 and FTL_0778 and its sequence does not overlap those of the flanking genes, indicating that its target(s) is located in trans. Base-pairing between a sRNA and target mRNA generally leads to changed translation and often the stability of the mRNA is affected as well. Therefore, to identify possible targets of Fvr1, we compared the transcriptomes of the LVS?fvr1(LVS, for Live Vaccine Strain) and LVS/pfvr1+ strains after growth in liquid broth.