Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling by array of serum microRNAs in rat livers to screen for specific biomarkers to be used in diagnosis of compound- and herb-induced liver injury.

ABSTRACT: In this study, we employed a strategy to screen miRNA expression profiles in liver tissue by miRCURY LNA(tm) microRNA array analysis followed by TaqMan probe-based quantitative reverse transcription-PCR (qRT-PCR) to validate the miRNA expression profiles in serum and liver of two parallel rat drug-induced liver injury (DILI) models induced by a compound (acetaminophen, APAP) or an herb (Dioscorea bulbifera, DB).

ORGANISM(S): Rattus norvegicus

SUBMITTER: Yuwen Su

PROVIDER: E-MEXP-3364 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A panel of serum microRNAs as specific biomarkers for diagnosis of compound- and herb-induced liver injury in rats.

Su Yu-Wen YW Chen Xi X Jiang Zhen-Zhou ZZ Wang Tao T Wang Cheng C Zhang Yun Y Wen Jing J Xue Mei M Zhu Dan D Zhang Yue Y Su Yi-Jing YJ Xing Tong-Yue TY Zhang Chen-Yu CY Zhang Lu-Yong LY

PloS one 20120518 5

<h4>Background</h4>Drug-induced liver injury (DILI) has been a public, economic and pharmaceutical issue for many years. Enormous effort has been made for discovering and developing novel biomarkers for diagnosing and monitoring both clinical and preclinical DILI at an early stage, though progress has been relatively slow. Additionally, herb-induced liver injury is an emerging cause of liver disease because herbal medicines are increasingly being used worldwide. Recently, circulating microRNAs ( ...[more]

PMID: 22624025

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Project description:Phenotypic plasticity, the ability of one genotype to express different phenotypes in response to changing environmental conditions, is one of the most common phenomena characterising the living world and is not only relevant for the ecology but also for the evolution of species. Daphnia, the waterflea, is a textbook example for predator induced phenotypic plastic defences including changes in life-history, behaviour and morphology. However, the analysis of molecular mechanisms underlying these inducible defences is still in its early stages.<br><br>We exposed Daphnia magna to chemical cues of the predator Triops cancriformis to identify key processes underlying plastic defensive trait formation. D. magna is known to develop an array of morphological changes in the presence of T. cancriformis including changes of carapace morphology and cuticle hardening. To get a more comprehensive idea of this phenomenon, we studied four different genotypes originating from habitats with different predation history, reaching from predator-free to temporary habitats containing T. cancriformis.<br><br>We analysed the morphologies as well as proteomes of predator-exposed and control animals. Three genotypes showed morphological changes when the predator was present. Using a high-throughput proteomics approach, we found 294 proteins which were significantly altered in their abundance after predator exposure in a general or genotype dependant manner. Proteins connected to genotype dependant responses were related to the cuticle, protein synthesis and calcium binding whereas the yolk protein vitellogenin increased in abundance in all genotypes, indicating their involvement in a more general response. Furthermore, genotype dependant responses at the proteome level correlated well with local adaptation to Triops predation.<br><br>Altogether, our study provides new insights concerning genotype dependant and general molecular processes involved in predator-induced phenotypic plasticity in D. magna.

Dataset Information

Transcription profiling by array of serum microRNAs in rat livers to screen for specific biomarkers to be used in diagnosis of compound- and herb-induced liver injury.

Publications

A panel of serum microRNAs as specific biomarkers for diagnosis of compound- and herb-induced liver injury in rats.

Similar Datasets

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