Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptional profiling of DEX-inducible SNRK3.15 seedlings in the presence of ABA

ABSTRACT: Transcriptional profiling of a DEX-inducible SNRK3.15 seedlings in the presence of ABA.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Shigeo Toh

PROVIDER: E-MEXP-3397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis.

Lumba Shelley S Toh Shigeo S Handfield Louis-François LF Swan Michael M Liu Raymond R Youn Ji-Young JY Cutler Sean R SR Subramaniam Rajagopal R Provart Nicholas N Moses Alan A Desveaux Darrell D McCourt Peter P

Developmental cell 20140501 3

The sesquiterpenoid abscisic acid (ABA) mediates an assortment of responses across a variety of kingdoms including both higher plants and animals. In plants, where most is known, a linear core ABA signaling pathway has been identified. However, the complexity of ABA-dependent gene expression suggests that ABA functions through an intricate network. Here, using systems biology approaches that focused on genes transcriptionally regulated by ABA, we defined an ABA signaling network of over 500 inte ...[more]

PMID: 24823379

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Project description:Abscisic acid (ABA) is a key hormone for plant growth, development and stress adaptation. Perception of ABA through four types of receptors has been reported. We show here that impairment of ABA perception through the PYR/PYL/RCAR branch reduces vegetative growth and seed production, and leads to a severely open stomata and dramatic ABA insensitive phenotype, even though other branches for ABA perception remain functional. Arabidopsis sextuple mutant impaired in 6 PYR/PYL receptors, namely PYR1, PYL1, PYL2, PYL4, PYL5 and PYL8, was able to germinate and grow even on 100 mM ABA. Whole-rosette stomatal conductance (Gst) measurements revealed that leaf transpiration in the sextuple pyr/pyl mutant was higher than in the ABA-deficient aba3-1 or ABA-insensitive snrk2.6 mutants. The gradually increasing Gst values of plants lacking three, four, five and six PYR/PYLs indicate quantitative regulation of stomatal aperture by this family of receptors. The sextuple mutant lacked ABA-mediated activation of SnRK2s and ABA-responsive gene expression was dramatically impaired as was reported in snrk2.2/2.3/2.6. In summary, these results show that ABA perception by PYR/PYLs plays a major role to regulate seed germination and establishment, basal ABA signaling required for vegetative and reproductive growth, stomatal aperture and transcriptional response to the hormone. Four biological replicates were generated for the 3 genotypes, pyr1pyl1pyl2pyl4pyl5pyl8 sextuple mutant, snrk2.2/2.3/2.6 triple mutant, and Col-0. All the samples were treated with ABA for 3 hours before harvesting. The four Col-0 samples were mix to create an unique reference Col-0 sample. Two comparasion were made, fisrt one, PYR/PYL sextuple mutant versus Col-0 reference sample and second one, SnRK2 triple mutant versus Col-0 reference sample. In each comparasion four biological replicates were made. Replicas number 1 and 2 were labeled with Cy5 for the mutant sample and Cy3 for the Col-0 reference sample, while the other two replicas,#3 and #4, were reversed-labeled.

Dataset Information

Transcriptional profiling of DEX-inducible SNRK3.15 seedlings in the presence of ABA

Publications

A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis.

Similar Datasets

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