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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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SiRNA ELK1 depletion and EGF stimulation

ABSTRACT: ELK1 is a well-known target of the ERK branch (EGF-responsive) of the MAP kinase pathway. This transcription profiling experiment studied the effects of ELK1 depletion by siRNA and subsequent EGF stimulation.

ORGANISM(S): Homo sapiens

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-3407 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ELK1 uses different DNA binding modes to regulate functionally distinct classes of target genes.

Odrowaz Zaneta Z Sharrocks Andrew D AD

PLoS genetics 20120510 5

Eukaryotic transcription factors are grouped into families and, due to their similar DNA binding domains, often have the potential to bind to the same genomic regions. This can lead to redundancy at the level of DNA binding, and mechanisms are required to generate specific functional outcomes that enable distinct gene expression programmes to be controlled by a particular transcription factor. Here we used ChIP-seq to uncover two distinct binding modes for the ETS transcription factor ELK1. In o ...[more]

PMID: 22589737

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