Transcriptional profiling by array of human primary keratinocytes to find new genes regulated by p21

ABSTRACT: Our goal is to find new genes regulated by p21 in human primary cells . To get it we carried out a gene expression profiling in two different models, human myeloid leukemia K562 cells and human keratinocytes both of them with conditional expression of p21. In order to identify genes specifically modulated by p21 we compared with the cell line with overexpression of p27, because p21 and p27 belong to the same gene family and regulated the same genes specially in cell cycle. So, our intention is to identify only genes regulated by p21 and not p27. In order to confirm these results we studied the p21-dependent repression of mitotic genes in a different cellular system. We chose human primary keratinocytes because they are non-tumorigenic, non-immortalized and epithelial cells, in contrast to

ORGANISM(S): Homo sapiens

SUBMITTER: NURIA FERRANDIZ 

PROVIDER: E-MEXP-3430 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress