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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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An Artificial miRNA Against HSPE Suppresses Melanoma Invasion Properties Correlating with a Down-regulation of Chemokines and MAPK Phosphorylation

ABSTRACT: Heparanase (HPSE) is an endo-beta-glucuronidase that can cleave heparan sulfate proteoglycans within extracellular matrix, basement membrane or on the cellular surface, closely linked with proliferation, migration, invasion and angiogenesis of tumor cells, and has become a hot treatment target in anti-cancer metastasis. In order to explore the probable mechanism of HPSE-miRNA on melanoma metastasis and to distinguish whether the silence effect on unpredicted genes was due to off-target effect of RNAi, we analyzed and compared genes between the Neg-miRNA transfected A375 cells and HPSE-miRNA1 or HPSE-miRNA2 transfected A375 cells by gene microarray analysis using OneArray(r) slides.

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaoyan Liu

PROVIDER: E-MEXP-3443 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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An artificial miRNA against HPSE suppresses melanoma invasion properties, correlating with a down-regulation of chemokines and MAPK phosphorylation.

Liu Xiaoyan X Fang Hong H Chen Hongchao H Jiang Xiaoling X Fang Deren D Wang Yan Y Zhu Dingxian D

PloS one 20120615 6

Ribonucleic acid interference (RNAi) based on microRNA (miRNA) context may provide an efficient and safe therapeutic knockdown effect and can be driven by ribonucleic acid polymerase II (RNAP II). In this study, we designed and synthesized miR155-based artificial miRNAs against heparanase (HPSE) constructed with BLOCK-iT™ Pol II miR RNAi Expression Vector Kit. The expression levels of HPSE declined significantly in both the mRNA and protein levels in HPSE-miRNA transfected melanoma cells that ex ...[more]

PMID: 22719918

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