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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Carvacrol treated Dentritic Cells

ABSTRACT: Tolerogenic dendritic cells (DCs) induce regulatory T-cells and dampen pathogenic T-cell responses. Hereby, they have gained interest as a therapeutic target in the combat against autoimmune diseases. In this study we investigated whether tolerogenic DCs are induced by the phytonutrient carvacrol, a molecule with known anti-inflammatory properties. In this study, bone marrow derived DCs were treated with carvacrol in combination with thermal stress. Gene expression profiles were obtained by microarray analysis to test for an induced tolerogenic phenotype. To investigate the tolerogenic properties of treated DCs in vivo, T-cell anergy or the induction of a regulatory T-cell phenotype was studied in antigen specific T-cells. Finally, treated DCs were tested by transfer into an experimental arthritis model.

ORGANISM(S): Mus musculus

SUBMITTER: Maria Foti

PROVIDER: E-MEXP-3489 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tolerogenic dendritic cells that inhibit autoimmune arthritis can be induced by a combination of carvacrol and thermal stress.

Spiering Rachel R van der Zee Ruurd R Wagenaar Josée J Kapetis Dimos D Zolezzi Francesca F van Eden Willem W Broere Femke F

PloS one 20120925 9

Tolerogenic dendritic cells (DCs) can induce regulatory T cells and dampen pathogenic T cell responses. Therefore, they are possible therapeutic targets in autoimmune diseases. In this study we investigated whether mouse tolerogenic DCs are induced by the phytonutrient carvacrol, a molecule with known anti-inflammatory properties, in combination with a physiological stress. We show that treatment of DCs with carvacrol and thermal stress led to the mRNA expression of both pro- and anti-inflammato ...[more]

PMID: 23050016

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