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Deregulated Myc expression induces addiction to AMPK-related kinase 5

ABSTRACT: U2OS cells were infected with shRNA vector targeting Ark5 or control vector, selected on puromycin for 48hrs, seeded on 5cm dishes at 20% confluency and harvested in triplicate 48hrs later

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Krause

PROVIDER: E-MEXP-3543 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deregulated MYC expression induces dependence upon AMPK-related kinase 5.

Liu Lidan L Ulbrich Jannes J Müller Judith J Wüstefeld Torsten T Aeberhard Lukas L Kress Theresia R TR Muthalagu Nathiya N Rycak Lukas L Rudalska Ramona R Moll Roland R Kempa Stefan S Zender Lars L Eilers Martin M Murphy Daniel J DJ

Nature 20120328 7391

Deregulated expression of the MYC oncoprotein contributes to the genesis of many human tumours, yet strategies to exploit this for a rational tumour therapy are scarce. MYC promotes cell growth and proliferation, and alters cellular metabolism to enhance the provision of precursors for phospholipids and cellular macromolecules. Here we show in human and murine cell lines that oncogenic levels of MYC establish a dependence on AMPK-related kinase 5 (ARK5; also known as NUAK1) for maintaining metab ...[more]

PMID: 22460906

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