Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Grf7-1


ABSTRACT: Arabidopsis thaliana (L.) Heynh. ecotype Columbia was used in this study. Seeds were sterilized and stratified at 4 C for 2 days in the dark, sown, and grown on germination media (GM) agar plates in a growth chamber with 16 hours of fluorescent light at 40M-110 ?mol m-2 sec-1, 22 C, and 70% relative humidity.

The T-DNA insertion line for GRF7 was obtained from the ABRC (Stock No. CS878963;? Sessions et al., 2002). The T-DNA insertion was confirmed by amplifying the LB-flanking region in genomic DNA of individual candidate lines.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kyonoshin Maruyama 

PROVIDER: E-MEXP-3559 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Arabidopsis growth-regulating factor7 functions as a transcriptional repressor of abscisic acid- and osmotic stress-responsive genes, including DREB2A.

Kim June-Sik JS   Mizoi Junya J   Kidokoro Satoshi S   Maruyama Kyonoshin K   Nakajima Jun J   Nakashima Kazuo K   Mitsuda Nobutaka N   Takiguchi Yuko Y   Ohme-Takagi Masaru M   Kondou Youichi Y   Yoshizumi Takeshi T   Matsui Minami M   Shinozaki Kazuo K   Yamaguchi-Shinozaki Kazuko K  

The Plant cell 20120831 8


Arabidopsis thaliana DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) functions as a transcriptional activator that increases tolerance to osmotic and heat stresses; however, its expression also leads to growth retardation and reduced reproduction. To avoid these adverse effects, the expression of DREB2A is predicted to be tightly regulated. We identified a short promoter region of DREB2A that represses its expression under nonstress conditions. Yeast one-hybrid screening for interactin  ...[more]

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