Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Silva_UMinho_Initial translational response of Saccharomyces cerevisiae to acetic acid-induced apoptosis

ABSTRACT: The initial translational response of the yeast Saccharomyces cerevisiae in response to acetic acid-induced apoptosis was investigated by microarray profiling of mRNAs contained in polysomal fractions obtained upon 0 (used as control), 15 and 30 minutes of acetic acid treatment. The mRNA fraction thus investigated corresponds to the mRNAs capable of overcoming the inhibition of cap-mediated translation initiation.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Laura Carreto

PROVIDER: E-MEXP-3570 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Involvement of yeast HSP90 isoforms in response to stress and cell death induced by acetic acid.

Silva Alexandra A Sampaio-Marques Belém B Fernandes Angela A Fernandes Angela A Carreto Laura L Rodrigues Fernando F Holcik Martin M Santos Manuel A S MA Ludovico Paula P

PloS one 20130815 8

Acetic acid-induced apoptosis in yeast is accompanied by an impairment of the general protein synthesis machinery, yet paradoxically also by the up-regulation of the two isoforms of the heat shock protein 90 (HSP90) chaperone family, Hsc82p and Hsp82p. Herein, we show that impairment of cap-dependent translation initiation induced by acetic acid is caused by the phosphorylation and inactivation of eIF2α by Gcn2p kinase. A microarray analysis of polysome-associated mRNAs engaged in translation in ...[more]

PMID: 23967187

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Project description:Integrated breeding strategies are used to increase both the yield potential and stability of crops. Most of these approaches have a direct genetic basis. The utility of epigenetics in breeding to improve complex traits such as yield and stress tolerance is not clear. A better understanding of the status of the epigenome and its contribution to the agronomic performance would help in developing strategies to incorporate the epigenetic component of complex traits in breeding,Starting from isogenic canola lines, epilines were generated by selecting recursively during three generations for lines with a higher energy use efficiency and drought tolerance. These epilines were more energy use efficient, drought tolerant, high nitrogen use efficient, and higher yielding under suboptimal conditions. Moreover, these characteristics were transgenerational inheritable. Transcriptome comparison with a line selected for energy use efficiency only revealed common differentially expressed genes related to the onset of signaling events regulating stress tolerance. Genes related to salt, osmotic, abscisic acid and drought were specifically differentially expressed in the drought tolerant epilines. The status of the epigenome, scored as differential trimethylation of lysine 4 of histone 3, supports the energy use efficient and drought tolerant phenotype by facilitating transcription of the genes that are found to be differentially expressed.From these results it can be concluded that the epigenome can be shaped by selection to increase yield and stress tolerance. This acquired knowledge will support further development of strategies to incorporate epigenetics in breeding. SRA study accession: SRP052946, Bioproject: PRJNA273932. Two canola (Brassica napus L. spp. oleifera (Metzg) Sinsk. f. biennis) epilines with low respiration and high NAD(P)H content were selected. Grinded leaf material from 26 day-old control and both epilines was collected in triplicate and served as input for deep sequencing on Illumina GAIIx.

Dataset Information

Silva_UMinho_Initial translational response of Saccharomyces cerevisiae to acetic acid-induced apoptosis

Publications

Involvement of yeast HSP90 isoforms in response to stress and cell death induced by acetic acid.

Similar Datasets

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