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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of Lotus japonicus wildtype and Ljgln2-2 mutant plants to investigate photorespiration

ABSTRACT: Wildtype and mutant plants lacking the plastidic isoform of glutamine synthetase were grown under CO2-enriched atmosphere and then transferred to low CO2 conditions. Transcriptomic profiling was carried out for plants under CO2-enriched atmosphere and plants after 2 days of transfer to low CO2 conditions.

ORGANISM(S): Lotus japonicus

SUBMITTER: Marco Betti

PROVIDER: E-MEXP-3603 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptomic and metabolic changes associated with photorespiratory ammonium accumulation in the model legume Lotus japonicus.

Pérez-Delgado Carmen M CM García-Calderón Margarita M Sánchez Diego H DH Udvardi Michael K MK Kopka Joachim J Márquez Antonio J AJ Betti Marco M

Plant physiology 20130606 4

The transcriptomic and metabolic consequences of the lack of plastidic glutamine (Gln) synthetase in the model legume Lotus japonicus were investigated. Wild-type and mutant plants lacking the plastidic isoform of Gln synthetase were grown in conditions that suppress photorespiration and then transferred for different lengths of time to photorespiratory conditions. Transcript and metabolite levels were determined at the different time points considered. Under photorespiratory active conditions, ...[more]

PMID: 23743713

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Transcription profiling by array of Lotus japonicus leaves from wild type and Ljgln2-2 mutant plants exposed to drought.

Project description:L. japonicus (Regel) Larsen cv, Gifu were initially obtained from Prof. Jens Stougaard (University Aarhus, Denmark) and then self-propagated at the University of Seville. Ljgln2-2 mutant was isolated from photorespiratory mutant screening as described previously (Orea et al., 2002; Mï¿½rquez et al., 2005; Betti et al., 2006). The mutant progeny of two consecutive back-crosses with WT was used for the present work. WT and mutant seeds were scarified and surface-sterilized, germinated in 1% agar Petri dishes, and transferred to pots using a 1:1 (v/v) mixture of vermiculite and sand as solid support. Five seedlings were planted in each pot and grown during 35 days in a growth chamber under 16/8 hours day/night, 20/18ï¿½C, with a photosynthetic photon flux density of 250 ï¿½mol/m2ï¿½s and a constant humidity of 70%. CO2 was automatically injected to a final concentration of 0.7% (v/v), to allow for normal growth of Ljgln2-2 mutant in a photorespiration suppressed atmosphere. Plants were watered with Hornum nutrient solution (Handberg & Stougaard, 1992). Drought was applied withholding irrigation for the reported period of time and sample plants or leaves were harvested for further analysis.

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