Flenniken_BeeArray2_2012

ABSTRACT: Flenniken - Honey bee gene expression microarray experimental design
To minimize variability between samples all arrayed bees were obtained from a single brood comb from a naturally mated queen, therefore all the bees were age-matched half-sisters. The bees selected for microarray analysis of virus (Sindbis-eGFP) co-injected with either virus-specific-dsRNA (vs-dsRNA) or non-specific dsRNA (ns-dsRNA) exhibited the reduced virus phenotype that was seen in the majority of the bees assayed. The five representative bees from each condition (v, v+vs-dsRNA, v+ns-dsRNA, dsRNA, and mock/injected with buffer) selected for microarray analysis were free of pre-existing conditions (assessed by APM analysis) (Runckel, Flenniken et al., 2011). To facilitate gene expression comparisons between multipl

ORGANISM(S): Apis mellifera

DISEASE(S): sindbis virus + non-specific RNA

SUBMITTER: Michelle Flenniken 

PROVIDER: E-MEXP-3608 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress