Drosophila Johnston organ genes

ABSTRACT: Hemizygous ato[1]/Df(3R)p[13] null mutants lack Johnston organ (JO) in their 2nd antennal segment. We screened for genes that are expressed in JO by comparing the 2nd antennal segment transcriptomes between ato[1]/Df(3R)p[13] mutants and balanced Df(3R)p[13]/TM3 and ato[1]/TM3 controls. To assess transcriptomes, we isolated the 2nd antennal segments of ~50 flies per strain and extracted their total RNA. Because about half of the JO cells are sensory neurons, we also isolated RNA from the brains of ato[1]/TM3 controls to delineate neuronal JO genes. cRNA was hybridized to Affymetrix Drosophila Genome 2.0 arrays. For each experiment, three biological replicates were run.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Martin Gopfert 

PROVIDER: E-MEXP-3609 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress