Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of S. cerevisiae haploid Sigma1278b strains with msa1 or msa2 or tec1 deletions

ABSTRACT: We determined and classified Msa1-, Msa2- and Tec1-regulated genes in haploid strains of the Sigma1278b genetic background under vegetative growth conditions. For this purpose, we measured transcriptional profiles of five different haploid MATa yeast strains of the following relevant genotypes: (JvdF_94 = YHUM1694) control; (JvdF_64 = YHUM1864) msa1-delta; (JvdF_65 = YHUM1865) msa2-delta; (JvdF_19 = YHUM1900) msa1-delta msa2-delta; (JvdF_17 = YHUM1700) tec1-delta. All strains were grown in duplicate in SC medium lacking leucine and histidine at 30 degrees C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Julia van der Felden 

PROVIDER: E-MEXP-3619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcription factors Tec1 and Ste12 interact with coregulators Msa1 and Msa2 to activate adhesion and multicellular development.

van der Felden Julia J   Weisser Sarah S   Brückner Stefan S   Lenz Peter P   Mösch Hans-Ulrich HU  

Molecular and cellular biology 20140414 12

In Saccharomyces cerevisiae and related yeast species, the TEA transcription factor Tec1, together with a second transcription factor, Ste12, controls development, including cell adhesion and filament formation. Tec1-Ste12 complexes control target genes through Tec1 binding sites (TEA consensus sequences [TCSs]) that can be further combined with Ste12 binding sites (pheromone response elements [PREs]) for cooperative DNA binding. The activity of Tec1-Ste12 complexes is known to be under negative  ...[more]

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