Transcription profiling by array of honey bee queens Apis mellifera L. to investigate the differential effects of insemination volume and substance on reproductive changes

ABSTRACT: Mating causes dramatic changes in female insects at the behavioral, physiological, and molecular levels. The factors driving these changes (e.g., seminal proteins, seminal volume) and the molecular pathways by which these factors are operating have been characterized only in a handful of insect species. Here we use instrumental insemination of honey bee queens to examine the role of the insemination substance (saline vs. semen) and volume (1 vs. 8 uL) in triggering post-mating changes. We also examine differences in gene expression patterns in the fat bodies of queens that have high ovary activation to determine if events during copulation can cause long-term changes in gene expression. We found that the instrumental insemination procedure alone caused cessation of mating flights and tr

ORGANISM(S): Apis mellifera

SUBMITTER: Elina Lastro 

PROVIDER: E-MEXP-3681 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress