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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in the thymus medulla in the absence and presence of acute graft-versus-host disease

ABSTRACT: To investigate gene expression profile of medullary thymic epithelial cells with high surface density of MHC class II (mTEChigh), a murine parent into F1 hematopoietic stem cell transplantation model was administered. As recipient mice (B6xDBA/2)F1 (B6D2F1) were used that received either a syngeneic transplant from a (B6xDBA/2)F1 mouse or an allogeneic transplant from a B6 mouse, which leads to acute graft-versus-host disease (aGVHD). mTEChigh (CD45-EpCAM+Ly51-UEA1+MHCIIhigh) are sorted by FACS.

ORGANISM(S): Mus musculus

SUBMITTER: Simone Dertschnig

PROVIDER: E-MEXP-3745 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epithelial cytoprotection sustains ectopic expression of tissue-restricted antigens in the thymus during murine acute GVHD.

Dertschnig Simone S Nusspaumer Gretel G Ivanek Robert R Hauri-Hohl Mathias M MM Holländer Georg A GA Krenger Werner W

Blood 20130529 5

Development of acute graft-versus-host disease (aGVHD) predisposes to chronic GVHD with autoimmune manifestations. A characteristic of experimental aGVHD is the de novo generation of autoreactive T cells. Central tolerance is dependent on the intrathymic expression of tissue-restricted peripheral self-antigens (TRA), which is in mature medullary thymic epithelial cells (mTEC(high)) partly controlled by the autoimmune regulator (Aire). Because TECs are targets of donor T-cell alloimmunity, we tes ...[more]

PMID: 23719300

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Leukocyte accumulation in graft blood vessels during self-limiting acute rejection of rat kidneys Immunobiology

Project description:Acute rejection episodes trigger chronic renal allograft vasculopathy. Numerous leukocytes, predominantly monocytes, accumulate in graft blood vessels during reversible acute rejection preceding chronic rejection of rat kidneys. We speculate that they contribute to transplant vasculopathy and set out to characterize them. Allogeneic renal transplantation was performed in the Fischer 344 to Lewis rat strain combination, Lewis isografts served as controls. Leukocytes were harvested by intensive perfusion of graft blood vessels and subjected to flow cytometry, quantitative RT-PCR and genome-wide transcriptional profiling. Kidneys of LEW and F344 rats were transplanted in LEW rats. Five biological replicates were performed for both isogenic and allogenic transplantation. Transcriptomes of allogenics were compared to isogenics on 5 dual-color hybridizations.

2010-09-24 | E-GEOD-18266 | biostudies-arrayexpress

Redefining thymus medulla specialization for central tolerance.

Project description: Not available

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The effect of cortex/medulla proportions on molecular diagnoses in kidney transplant biopsies: rejection and injury can be assessed in medulla

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2017-04-27 | E-GEOD-95611 | biostudies-arrayexpress

promiscuous gene expression in the mouse thymus

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Subclinical effects of remote ischaemic conditioning in human kidney transplants revealed by quantitative proteomics

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2021-09-09 | PXD019284 | Pride

Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution.

Project description: Not available

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RNA-seq of bronchoalveolar lavage samples of lung transplant primary graft dysfunction

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2023-02-15 | E-MTAB-12282 | biostudies-arrayexpress

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Project description: Not available

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Transcription profiling of mouse acute lymphoblastic leukemia in vivo after allogeneic or syngeneic bone marrow transplantation

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