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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Sumoylation at Chromatin Governs Coordinated Repression of a Transcriptionnal Program Essential for Cell Growth and Proliferation

ABSTRACT: mRNA profiling of WI38 wild-type or overexpressiong the SUMO E3 ligase PIASy in human primary fibroblasts 24h post-infection. The goal of this study is to analyse transcriptional changes in cells over-expressing the SUMO E3 ligase PIASy and to compare them with ChIPseq data for several histones marks and proteins of the SUMO machinery including PIASy

ORGANISM(S): Homo sapiens

SUBMITTER: guillaume soubigou

PROVIDER: E-MEXP-3776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sumoylation at chromatin governs coordinated repression of a transcriptional program essential for cell growth and proliferation.

Neyret-Kahn Hélène H Benhamed Moussa M Ye Tao T Le Gras Stéphanie S Cossec Jack-Christophe JC Lapaquette Pierre P Bischof Oliver O Ouspenskaia Maia M Dasso Mary M Seeler Jacob J Davidson Irwin I Dejean Anne A

Genome research 20130726 10

Despite numerous studies on specific sumoylated transcriptional regulators, the global role of SUMO on chromatin in relation to transcription regulation remains largely unknown. Here, we determined the genome-wide localization of SUMO1 and SUMO2/3, as well as of UBC9 (encoded by UBE2I) and PIASY (encoded by PIAS4), two markers for active sumoylation, along with Pol II and histone marks in proliferating versus senescent human fibroblasts together with gene expression profiling. We found that, whe ...[more]

PMID: 23893515

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Sumoylation at Chromatin Governs Coordinated Repression of a Transcriptionnal Program Essential for Cell Growth and Proliferation 3

Project description:mRNA profiling of WI38 expressing shCt or shUbc9 4 days post-infection. The goals of this study is to analyse transcriptionnal changes in cells were sumoylation pathway is inactivated and to compare them with ChIPseq data for several histones marks and proteins of the SUMO machinery.

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