Transcription profiling of mosquitos fed blood infected with two alternative P. berghei strains: wild type (wt) or an invasion-deficient, CTRP (Circumsporozoite- and TRAP-related protein) knockout (ko) strain

ABSTRACT: Female mosquitoes fed with blood infected with two alternative P. berghei strains: wild type (wt) or an invasion-deficient, CTRP (Circumsporozoite- and TRAP-related protein) knockout (ko) strain. We used CTRPko-infected midguts as reference, as they experience pre-invasion conditions similar to those of wt-infected midguts. Therefore, wt/CTRPko comparisons should reveal transcriptional changes specifically associated with parasite midgut invasion. We collected midguts at 18-22 h post infection (B, before midgut invasion), 24-28 h (I; during invasion) and 40-44 h (A; after invasion) and used them to hybridize two DNA microarrays: 4K microarrays containing 3,840 EST clones and MMC1 (or 20K) microarrays containing the same plus 15,840 additional ESTs. Two different biological experiments and

ORGANISM(S): Anopheles gambiae

DISEASE(S): Plasmodium berghei CTRP knockout strain (Dessens et al., 1999) infection

SUBMITTER: Fotis C. Kafatos 

PROVIDER: E-MEXP-378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress