Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

14 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gamma-Catenin is expressed throughout normal human hematopoietic development and is required for normal PU.1-dependent monocyte differentiation

ABSTRACT: CD34+ HPC cells were retrovirally transduced with either control PINCO-GFP or PINCO Gamma-Catenin-GFP and analysed for Affymetrix mRNA expression. Gamma-Catenin and control matched transduced cells were also cultured until day 6 and were fractionated into the main progenitor groups for Affymetrix microarray analysis as previously described (tonks 2007). Briefly, a depletion strategy using the Miltenyi Biotec MiniMACS system was used for this purpose where, myeloblasts (CD15+) were enriched from mixed lineage transduced cells by serial depletion of monoblasts (CD14hi) and erythroblasts (CD36+) using immunomagnetic beads directed against those antigens.

ORGANISM(S): Homo sapiens

SUBMITTER: Alex Tonks

PROVIDER: E-MEXP-3810 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human normal progenitor cells mutated for RUNX1-RUNX1 during myeloid and erythroid development to identify genes disregulated by RUNX1-RUNX1

Project description:In order to identify genes dysregulated by the aberrant transcriptional activity of RUNX1-RUNX1T1, we used microarrays to determine the effect of this mutation on gene expression during myeloid and erythroid development of normal human progenitor cells.

2007-10-01 | E-MEXP-583 | biostudies-arrayexpress

Transcription profiling of human T cell leukaemia cell line Jurkat that were retrovirally transduced with constitutievely active forms of Notch to identify novel transcriptional targets of Notch signalling

Project description:In this study we aimed to identify novel transcriptional targets of Notch signalling in the T cell leukaemia cell line, Jurkat. RNA was prepared from Jurkat cells retrovirally transduced with an empty vector (Mock) or vectors containing constitutively active forms of Notch (N1deltaE or N3deltaE), and used for Affymetrix microarray analysis. As expected, several known transcriptional target of Notch, such as HES1 and HERP2, were found to be overexpressed in Notch-transduced cells, however, many novel transcriptional targets of Notch signalling were identified using this approach. These included the T cell costimulatory molecule CD28, the anti-apoptotic protein GIMAP5, and inhibitor of DNA binding 1 (1D1).

2009-07-15 | E-MEXP-1744 | biostudies-arrayexpress

Nurf301 isoform requirements

Project description:Comparison of wild-type and Nurf301 mutant third instar larvae. Nurf301 mutants that remove all Nurf301 isoforms (Null) or mutants that remove full-length Nurf301 isoforms (DeltaC) were used

2009-07-24 | E-MEXP-2011 | biostudies-arrayexpress

Transcription profiling of diagnostic tumoral samples in relation to primary response to drug treatment

Project description:Expression profile of diagnostic tumoral samples in relation to primary response to drug treatment

2005-10-30 | E-MEXP-388 | biostudies-arrayexpress

Transcription profiling of human indolent and aggressive forms of Chronic Myeloid Leukaemia (CML)

Project description:Title: Gene expression analysis of indolent and aggressive forms of Chronic Myeloid Leukaemia (CML). Description: Chronic Myeloid Leukaemia presents in chronic phase (CP) and terminates in 'blast crisis'. Despite a common abnormality, the duration of CP is variable. The aim is to compare the gene expression profiles of the indolent and aggressive forms of CML. All samples were taken within 3 months of first diagnosis. Indolent patients were defined by chronic phase CML, duration minimum 7 years. Aggressive patients were defined by chronic phase, duration maximum 3 years.

2013-01-30 | E-MIMR-17 | biostudies-arrayexpress

Transcription profiling of human nodal peripheral T-cell lymphomas

Project description:Microarray technology was used to monitor the level of expression of 7,657 human genes in a set of 35 nodal peripheral T-cell lymphomas.

2007-01-30 | E-TABM-191 | biostudies-arrayexpress

Transcription profiling by array of mouse liver from wild type and beta-catenin knockout mice before and after hepatectomy

Project description:Study of beta-catenin activation in liver regeneration using comparaison between ko-mice for beta-catenin gene with control mice before and after hepatectomy.

2010-07-27 | E-MEXP-2828 | biostudies-arrayexpress

Transcription profiling of patients with diffuse large B-cell lymphoma who do or do not respond to treatment to identify biological markers of sensivity to high-clinical-risk-adapted therapy

Project description:Microarray technology was used to identify new predictive biomarkers in samples of clinically homogeneus patients.

2008-10-26 | E-TABM-364 | biostudies-arrayexpress

Identification of hypoxia, HIF1 and HIF2 target genes in human CB CD34+ cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-03-01 | E-GEOD-54663 | biostudies-arrayexpress

Transcription profiling of N. gonorrhoeae nmb1650 knockout mutant and parental strains grown in capnoaerobic conditions

Project description:Comparison of N. gonorrhoeae nmb1650 knockout mutant and wild-type parent strains grown in capnoaerobic conditions (normal atmosphere +5% CO2)

2011-05-01 | E-MEXP-1664 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data