Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

8 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of transcription profile of the activation tagging mutant zfhd2-1D

ABSTRACT: zfhd2-1D is an activation tagging mutant was isolated in a screening of seed longevity. The aim of this project is to know the transcriptional changes that allow this kind of phenotype.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Eduardo Bueso

PROVIDER: E-MEXP-3857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of Arabidopsis thaliana seedlings wild type vs transgenic plants under drought stress

Project description:Plants engineered for abiotic stress tolerance may soon be commercialized. The engineering of these plants typically involves the manipulation of complex multigene networks and may therefore have a greater potential to introduce pleiotropic effects than the simple monogenic traits that currently dominate the plant biotechnology market. Drought- tolerant Arabidopsis thaliana were engineered through overexpression of the transcription factor ABF3 in order to investigate unintended pleiotropic effects. In order to eliminate position effects, the Cre/lox recombination system was used to create control plant lines that contain identical T-DNA insertion sites but with the ABF3 transgene excised. This additionally allowed us to determine if Cre recombinase can cause unintended effects that impact the transcriptome. Microarray analysis of control plant lines that underwent Cre-mediated excision of the ABF3 transgene revealed only two genes that were differentially expressed in more than one plant line, suggesting that the impact of Cre recombinase on the transcriptome was minimal. In the absence of drought stress, overexpression of ABF3 had no effect on the transcriptome, but following drought stress, differences were observed in the gene expression patterns of plants overexpressing ABF3 relative to control plants. Examination of the functional distribution of the differentially expressed genes revealed strong similarity indicating that unintended pathways were not activated. In response to drought stress, overexpression of ABF3 results in a reprogramming of the drought response, which is characterized by changes in the timing or strength of expression of some drought response genes, without activating any unexpected gene networks. These results illustrate that important gene networks are highly regulated in Arabidopsis and that engineering stress tolerance may not necessarily cause extensive changes to the transcriptome.

2010-01-28 | E-MEXP-2435 | biostudies-arrayexpress

Transcription profiling of Arabdopsis eto1 JMT mutants

Project description:Comparison of global gene expression of eto1 JMT under light or dark condition

2010-04-01 | E-MEXP-2182 | biostudies-arrayexpress

Col versus Col_Rose Bengal

Project description:MS treated wild-type Arabidopsis seedlings versus MS Rose Bengal (RB)-containing medium (10 microM) wild type treated seedlings

2011-03-01 | E-MEXP-3009 | biostudies-arrayexpress

Col_Rose Bengal versus eto1-14_Rose Bengal

Project description:Rose Bengal-treated wild-type seedlings versus Rose Bengal-treated eto1-14 mutant

2011-03-01 | E-MEXP-3011 | biostudies-arrayexpress

Col_Rose Bengal versus lox1lox5_Rose Bengal

Project description:Rose Bengal-treated wild type seedlings versus Rose Bengal-treated lox1lox5 mutant

2011-03-01 | E-MEXP-3010 | biostudies-arrayexpress

Transcription profiling by array of Arabidosis seeds after treatment with EL6 and GR24 to investigate the effect on germination

Project description:Effect of the chemical EL6 and GR24 on germination in Arabidopsis.

2008-10-21 | E-MEXP-1607 | biostudies-arrayexpress

AtOFP1 regulated genes

Project description:35S::AtOFP1-GR seeds were directly sown on MS/G plates without or with 10 uM DEX and stratified at 4C in dark for 2 days. Imbibed seeds were then transferred to growth conditions (23°C with 14/10 hr photoperiod at approximately 120 umol m-2 s-1) and grown for 7d.

2012-05-01 | E-MEXP-3222 | biostudies-arrayexpress

The embryonic leaf identity gene FUSCA3 also regulates vegetative phase transitions by negatively regulating ethylene action in Arabidopsis

Project description:The embryonic temporal regulator FUSCA3 (FUS3) plays major roles in the establishment of embryonic leaf identity and regulation of developmental timing. Loss-of-function mutations of this B3-domain transcription factor result in replacement of cotyledons with leaves and precocious germination, while constitutive misexpression causes the conversion of leaves into cotyledon-like organs and delays vegetative and reproductive phase transitions. To identify downstream targets of FUS3 involved in vegetative phase transitions, we performed microarray analysis on seedlings that transiently activate FUS3 using the AtML1:FUS3-GR-DEX inducible system. Using this construct, FUS3 is ectopically expressed in the ML1 or epidermal layer and becomes activated upon addition of dexamethasone. We found that activation of FUS3 after germination dampens the expression of genes involved in the biosynthesis and response to the plant hormone ethylene, while a loss-of-function fus3 mutant shows many phenotypes consistent with increased ethylene signaling. This FUS3-dependent regulation of ethylene signaling also impinges on timing functions outside of embryogenesis. Loss of FUS3 function results in accelerated vegetative phase change and this is again partially dependent on functional ethylene action. This alteration in vegetative phase transition is dependent on both embryonic and vegetative FUS3 function, suggesting that this important transcriptional regulator controls both embryonic and vegetative developmental timing. This study indicates that the embryonic regulator FUS3 not only regulates the embryonic-to-vegetative phase transition through hormonal regulation, but also functions postembryonically to modulate vegetative phase transitions by negatively regulating ethylene action.

2011-12-11 | E-MEXP-3465 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana AtCPL5 overexpressing seedlings

Project description:Wild-type and AtCPL5 overexpressing seedlings were compared

2010-01-20 | E-MEXP-2552 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis thaliana seedlings treated with CTL-VI or GR24

Project description:Effect of the chemical EL6 and GR24 on seedling growth in Arabidopsis

2010-01-06 | E-MEXP-2494 | biostudies-arrayexpress