Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

16 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

NLRP3 inflammasome affects DNA damage responses after oxidative and genotoxic stress in dendritic cells

ABSTRACT: Effect of Monosodium Urate Crystals in wild-type and NLRP3 ko murine dendritic cells

ORGANISM(S): Mus musculus

SUBMITTER: Alessandra Mortellaro

PROVIDER: E-MEXP-3858 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The NLRP3 inflammasome affects DNA damage responses after oxidative and genotoxic stress in dendritic cells.

Licandro Ginevra G Ling Khor Hwei H Beretta Ottavio O Lai Junyun J Derks Heidi H Laudisi Federica F Conforti-Andreoni Cristina C Liang Qian Hong H Teng Gim Gee GG Ricciardi-Castagnoli Paola P Mortellaro Alessandra A

European journal of immunology 20130524 8

The NOD-like receptor (NLR) family pyrin domain-containing 3 (NLRP3) inflammasome is a cytoplasmic protein complex that mediates inflammatory responses to a broad array of danger signals. The inflammasome drives caspase-1 activation and promotes secretion of the pro-inflammatory cytokines IL-1β and IL-18, and might also participate in other cellular processes. Here, we tried to identify new pathways regulated by the NLRP3 inflammasome in murine dendritic cells (DCs) in response to monosodium ura ...[more]

PMID: 23619996

Similar Datasets

Bone marrow derived dendritic cells (BMDC) and MDP and MSU

Project description:Muramyl dipeptide (MDP) and Monosodium urate crystals (MSU) promote a synergistic effect on NOD2 and NLRP3 with a unique transcriptional profile in murine dendritic cells

2010-12-01 | E-MEXP-2905 | biostudies-arrayexpress

Study of transcriptional effects of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line.

Project description:Aim of the study was to characterize at a molecular level (changes in transcriptomes) the effect of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line. This was adressed by using a culture model. The HaCaT cell line (human keratinocytes) was stimulated by MSU (1mg/mL) vs control for 12 hrs. By using genome-wide expression profiling, we identified deregulation of functionally relevant gene networks. HaCaT were obtained from Cell Lines Service (Eppelheim, Germany) and grown in DMEM medium (PAN biotech, Aidenbach, Germany) supplemented with 10% FBS (Life Technology, Grand Island, NY, USA), L-glutamine and non-essential amino acid. Before the treatment HaCaT cells were cultured in serum-free medium for 12hrs. HaCaT were treated with MSU (1mg/ml) vs DMEM control for 12hrs then submitted to RNA extration and gene expression profiling. Triplicate experiments were performed: HaCaT control (n=3), MSU-treated (n=3).

2015-10-29 | E-GEOD-65931 | biostudies-arrayexpress

The Ragulator complex regulates NLRP3 inflammasome activation through interaction with HDAC6

Project description:The activation of the NLRP3 inflammasome is spatiotemporally orchestrated by various organelles, but the precise roles of lysosomes are still unclear. Here we show the vital role of the Ragulator complex, a lysosomal protein, in NLRP3 inflammasome activation. Deficiency of Lamtor1, an essential component of the Ragulator complex, abrogated NLRP3 inflammasome activation in murine macrophage and human monocytic cells. Myeloid-specific Lamtor1-deficient mice showed remarkable attenuation of the severity of NLRP3-associated inflammatory diseases, including LPS-induced sepsis, alum-induced peritonitis, and monosodium urate (MSU)-induced arthritis. Mechanistically, Lamtor1 interacted with histone deacetylase 6 (HDAC6) during NLRP3 inflammasome activation, and this interaction augmented the interaction between the Ragulator complex and NLRP3. Lack of HDAC6 attenuated the interaction between Lamtor1 and NLRP3, resulting in insufficient NLRP3 inflammasome activation. Furthermore, DL-all-rac-α-tocopherol inhibited Lamtor1–HDAC6 interaction, resulting in diminished NLRP3 inflammasome activation. DL-all-rac-α-tocopherol alleviated acute gouty arthritis and MSU-induced peritonitis. Our results provide insight into the role of lysosomes in providing a platform for the activation of NLRP3 inflammasomes by the Ragulator complex.

2022-11-29 | PXD033497 | Pride

Transcription profiling by array of human peripheral blood monocytes stimulated with HbHp for 2 or 5 days

Project description:Peripheral human blood monocytes were stimulated with HbHp(2mg/ml) for 2 or 5 days.

2011-01-31 | E-MEXP-2820 | biostudies-arrayexpress

ChIP-chip by array of mouse haematopoietic and endothelial cell lines using an anti-H3 trimethyl lysine 4 antibody

Project description:chromatin immunoprecipitations were performed in mouse haematopoietic and endothelial cell lines using an anti-H3 trimethyl lysine 4 antibody

2011-09-06 | E-MEXP-2908 | biostudies-arrayexpress

Transcriptomic analysis of synovial fibroblasts from monosodium urate crystal-induced arthritis with or without I-BET151 treatment.

Project description:We used I-BET 151, an isoxazoloquinoline that specifically inhibits interaction of BET proteins with acetylated histones to restrict inflammatory tissue priming in a mouse model of iterated monosodium urate (MSU) crystal-induced arthritis. Systemic administration of I-BET 151 abolished the enhancement of arthritis upon repeated injection of MSU crystals.

2022-02-17 | GSE196679 | GEO

Transcription profiling by array of human B cell lymphoma cell lines in response to rituximab treatment

Project description:HF-1, Granta-519, SUDHL4,Oci-Ly10 and Oci-Ly3 cell lines, representing different B cell lymphoma subtypes, were induced with rituximab (R) for three hours and analyzed for differentially expressed genes compared to untreated control.

2010-06-22 | E-MEXP-2317 | biostudies-arrayexpress

Transcription profiling of human monocytes incubated with or without dexamethasone and with Hb-Hp for different time

Project description:Human Monocytes were incubated with or without dexamethasone for 36h. Then non-primed or dexamethasone-primed monocytes were incubated with 500ug/ml Hb-Hp for 0h, 8h and 18h. RNA of all samples was analyzed with Agilent whole genome 4x44k microarrays (dual-color). For factorial analysis all samples were individually analyzed using the same reference sample (no pre-treatment, 0h).

2010-07-31 | E-MEXP-2598 | biostudies-arrayexpress

Transcription profile of dendritic cell line D1 treated with Lactococcus Lactis

Project description:Effect of Lactococcuc Lactis (MOI 1:1000) on D1 dendritic cells on different time points: 0h, 4h, 8h, 24h

2012-03-28 | E-MEXP-3162 | biostudies-arrayexpress