Photoreceptor precursors derived from 3D cultures of embryonic stem cells mature and integrate in degenerate retina

ABSTRACT: To isolate photoreceptor precursors fit for transplantation, we adapted a 3D differentiation protocol that generates neuroretina from mouse ES cells. We used an adeno-associated viral vector (pseudotype2/9)carrying a GFP reporter under the control of a Rhodopsin promoter (AAV2/9.Rhop.GFP)to select rod precursors. AAV2/9.Rhop.GFP+ rods were sorted at days 26 and 34 and post natal day P12.

ORGANISM(S): Mus musculus

SUBMITTER: mike hubank 

PROVIDER: E-MEXP-3921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress