Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Tracheal immune response to bacteria in Drosophila

ABSTRACT: Drosophila larvae were infected with Erwinia bacteria (Ecc15) by introducing concentrated bacterial pellet into the fly medium. The tracheae were dissected 24h later. Three genotypes of larvae were compared: wt (CantonS), RelE20 and PGRP-LA, each present in unchallenged and infected conditions. 3 independent repeats were performed.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Mathilde Gendrin

PROVIDER: E-MEXP-3925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Functional analysis of PGRP-LA in Drosophila immunity.

Gendrin Mathilde M Zaidman-Rémy Anna A Broderick Nichole A NA Paredes Juan J Poidevin Mickaël M Roussel Alain A Lemaitre Bruno B

PloS one 20130726 7

PeptidoGlycan Recognition Proteins (PGRPs) are key regulators of the insect innate antibacterial response. Even if they have been intensively studied, some of them have yet unknown functions. Here, we present a functional analysis of PGRP-LA, an as yet uncharacterized Drosophila PGRP. The PGRP-LA gene is located in cluster with PGRP-LC and PGRP-LF, which encode a receptor and a negative regulator of the Imd pathway, respectively. Structure predictions indicate that PGRP-LA would not bind to pept ...[more]

PMID: 23922788

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Project description:The unsurpassed simplicity of the fruitflyâs airway epithelium, that is made of homogenous epithelial cells only, favours its use as a model to study general features and response characteristics of airway epithelia in general. All epithelial cells are able to launch an immune response as characterized by the expression of antimicrobial peptide genes. Infection induces a complex change in the expression profile of these epithelial cells. Outstanding are a priming of the immune system and the launch of a survival program, presumably to counteract infection induced apoptotic signals, which comprises the concurrent expression of known longevity genes such as dFoxo, and dThor. In regions of the airway epithelium with strong immune reactions, a complex remodelling of the airways can be observed, which is characterized by metaplasia and presumably also by hyperplasia of the affected epithelial cells. At the transcriptional level, this reorganization of the airway epithelium is mirrored by a recapitulation of genetic programs that are characteristic for early phases of airway development. Taken together, the response characteristics of the flyâs airway epithelium towards infections discloses features that are known from inflammatory diseases of the human lung, thus opening the opportunity to study fundamental aspects of these diseases in the fly. Keywords:infection, Erwinia c., third instar larva, airway epithelium, two-colour microarray Infection of the airway epithelium by the gram-negativ bacteria Erwinia carotovora. For the infection experiments third instar larvae of the GFP-reporter strain YW DD1 were used and only isolated when the whole epithelium of the airway epithelium showed GFP expression. Uninfected larvae were used as controls. In general, four replicates were performed including dye-swaps. A table of significantly-regulated genes from the SAM-output and GeneTraffic-output has been linked as a supplementary file at the foot of this record.

Dataset Information

Tracheal immune response to bacteria in Drosophila

Publications

Functional analysis of PGRP-LA in Drosophila immunity.

Similar Datasets

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