Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

11 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cytokeratin 15 marks basal epithelia in developing ureters and is upregulated in a subset of urothelial transitional cell carcinomas

ABSTRACT: The mammalian ureter contains a water-tight epithelium surrounded by smooth muscle. Key molecules have been defined which regulate ureteric bud initiation and drive the differentiation of ureteric mesenchyme into peristaltic smooth muscle. Less is known about mechanisms underlying the developmental patterning of the multilayered epithelium characterising the mature ureter. In skin, which also contains a multilayered epithelium, cytokeratin 15 (CK15), an intermediate filament protein, is a marker for cells whose progeny contribute to epidermal regeneration following wounding. Moreover, CK15+ precursor cells in skin can give rise to basal cell carcinomas. In the current study, using transcriptome microarrays of embryonic wild type mouse ureters, we detected Krt15, coding for CK15.

ORGANISM(S): Mus musculus

SUBMITTER: Fiona Marriage

PROVIDER: E-MEXP-3929 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcripts identified by microarray analysis that were deregulated in E15.5+6d Tbx18(cre/+);FGFR1(fl/fl);FGFR2(fl/fl) (Fgfr1/2cDKO) ureter cultures

Project description:Evaluation of the transcriptional changes in E15.5+6d ureter cultures upon loss of Fgfr1 and Fgfr2 in the ureteric mesenchyme.

2022-09-06 | GSE197368 | GEO

RNA-SEQ analysis of various developing and adult kidney components.

Project description:We used micro-dissection techniques and/or FACS to isolate cell types from the developing and adult kidney (E11.5 ureteric buds, E12.5, P1 and P4 cap mesenchyme, E15.5 collecting ducts, proximal tubules, ureter, Adult renal proximal tubules, podocytes, endothelial and mesangial cells). RNA-SEQ analysis was performed to determine the transcriptional profile of each cell type, identify component specific transcripts and isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Total RNA is obtained from micro-dissected and/or FACS isolated embryonic and adult kidney components. The long term goal is to generate a transcriptional atlas of developing kidney.

2015-01-27 | E-GEOD-64959 | biostudies-arrayexpress

Distal nephron plasticity allows the induction of ureteric tip and stalk for the modelling of collecting duct disease

Project description:During development, distinct progenitors contribute to the nephrons versus the ureteric epithelium of the kidney. Indeed, previous pluripotent stem cell-derived models of kidney tissue either contain nephrons or pattern specifically to the ureteric epithelium. By reanalysing the transcriptional distinction between distal nephron and ureteric epithelium in human fetal kidney, we show here that while existing nephron-containing kidney organoids contain distal nephron epithelium and no ureteric epithelium, this distal nephron segment alone displays significant in vitro plasticity and can adopt a ureteric epithelial tip identity when isolated and cultured in defined conditions. “Induced” ureteric epithelium cultures can be cryopreserved, serially passaged without loss of identity and transitioned towards a collecting duct fate. Indeed, cultures harbouring loss-of-function mutations in PKHD1 recapitulate the cystic phenotype associated with autosomal recessive polycystic kidney disease.

2021-01-15 | GSE161255 | GEO

Transcription profiling of hepatocytes from mice that are hyperglycaemic, obese and insulin resistant as a result of being fed a high fat diet

Project description:Effect of high fat diet feeding on gene expression

2006-11-15 | E-MEXP-893 | biostudies-arrayexpress

Transcription profiling of three strains of rat that are normoglycaemic or hyperglycaemic

Project description:Effects of hyperglycaemia and genetic background differences on gene expression in rats

2006-11-15 | E-MEXP-889 | biostudies-arrayexpress

Fat transcriptome patterns associated with heterogeneous metabolic, hormonal and behavioral adaptation to high fat diet feeding in mice

Project description:The effect of high fat diet feeding on adipose tissue gene transcription regulation was investigated in C57Bl/6J mice using Affymetrix gene expression arrays. Expression profiling was determined in 5 months old male mice showing heterogeneous metabolic, hormonal and behavioral adaptation to high fat diet (40% fat) feeding for 15 weeks. Control mice were fed a standard carbohydrate chow. Six animals per group were used.

2014-04-13 | E-MTAB-2569 | biostudies-arrayexpress

Transcription profiling of rat to investigate effects of hyperglycaemi and genetic background on gene expression

Project description:Effects of hyperglycaemia and genetic background differences on renal gene expression

2008-08-09 | E-MEXP-1195 | biostudies-arrayexpress

WTCHG - Mouse - Liver transcriptome adaptations to high fat diet

Project description:The effect of high fat diet feeding on liver gene transcription regulation was investigated in BALB/c mice using Affymetrix gene expression arrays. Expression data was determined in 5 months old male mice fed a high fat diet (40% fat) for 15 weeks. Control mice were fed a standard carbohydrate chow. Six animals per group were used.

2012-01-11 | E-MTAB-488 | biostudies-arrayexpress

Ablation of the epithelial-specific splicing factor Esrp1 results in ureteric branching defects and reduced nephron number

Project description:Evaluation of splicing in ureteric epithelium in Esrp1;Esrp2 double knockout mice compared to Esrp1+/+;Esrp2-/- control mice.

2016-05-21 | GSE81716 | GEO

Transcripts identified by microarray analysis that were deregulated in E13.5 Pax-2cre;Fgfr1(fl/?);Fgfr2(fl/fl) (Fgfr2cKO) ureters.

Project description:Evaluation of the transcriptional changes in E13.5 ureters upon loss of Fgfr2 (and reduction of Fgfr1) in the ureteric epithelium.

2022-01-04 | GSE178093 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data