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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNAi knock down of HectH9 in human osteosarcoma samples to identify myc regulated genes

ABSTRACT: Analysis of Gene Regulation by Myc in the absence of HectH9

ORGANISM(S): Homo sapiens

SUBMITTER: Federica Marinoni

PROVIDER: E-MEXP-406 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The ubiquitin ligase HectH9 regulates transcriptional activation by Myc and is essential for tumor cell proliferation.

Adhikary Sovana S Marinoni Federica F Hock Andreas A Hulleman Esther E Popov Nikita N Beier Rudi R Bernard Sandra S Quarto Micaela M Capra Maria M Goettig Stephan S Kogel Ulrike U Scheffner Martin M Helin Kristian K Eilers Martin M

Cell 20051101 3

The Myc oncoprotein forms a binary activating complex with its partner protein, Max, and a ternary repressive complex that, in addition to Max, contains the zinc finger protein Miz1. Here we show that the E3 ubiquitin ligase HectH9 ubiquitinates Myc in vivo and in vitro, forming a lysine 63-linked polyubiquitin chain. Miz1 inhibits this ubiquitination. HectH9-mediated ubiquitination of Myc is required for transactivation of multiple target genes, recruitment of the coactivator p300, and inductio ...[more]

PMID: 16269333

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