Transcription profiling of Arabidopsis with 35S:BatIMS transgene to analyse effects of IMPS overexpression
Ontology highlight
ABSTRACT:
INSTRUMENT(S): GeneChip Scanner 3000 [Affymetrix]
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Ben Field
PROVIDER: E-MEXP-424 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Expression of a Brassica isopropylmalate synthase gene in Arabidopsis perturbs both glucosinolate and amino acid metabolism.
Field Ben B Furniss Caroline C Wilkinson Andrew A Mithen Richard R
Plant molecular biology 20060301 5
Isopropylmalate synthase (IPMS) is a key enzyme in the biosynthesis of the essential amino acid leucine, and thus primary metabolism. In Arabidopsis, the functionally similar enzyme, methythiolalkylmalate synthase (MAM), is an important enzyme in the elongation of methionine prior to glucosinolate (GSL) biosynthesis, as part of secondary metabolism. We describe the cloning of an IPMS gene from Brassica, BatIMS, and its functional characterisation by heterologous expression in E. coli and Arabido ...[more]
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