Transcription profiling of mouse cells expressing tet inducible LhX2, withdrawal of LHX2 over time was analysed

ABSTRACT: Expression of the lim-homeodomain transcription factor is required for sustained maintenance of heamatopoietic stem cell like cells in undifferentiated form durng in vitro culturing. Cell lines were created from the mouse embryonic stem (ES) cell line, Ainv15. The gene Lhx2 was introducted into these cells under the control of a tetracycline-responsive element. In the presence of tetracycline (or its analogue doxycycline), these cells express Lhx2. In DoxHPC7 GFP is co-expressed together with the Lhx2 gene. We used these cells to carry out a time-course study where the effects of Lhx2 withdrawal were studied.

INSTRUMENT(S): G2565BA Microarray Scanner

ORGANISM(S): Mus musculus

SUBMITTER: Joakim Lunderberg 

PROVIDER: E-MEXP-431 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress